PDF(Pubmed)
Abstract：
Endothelial cells (ECs) maintain vessel tone and barrier integrity, regulate blood homeostasis, and prevent the extravasation of leukocytes under normal physiological conditions. Because of the limited lifespans and batch-to-batch differences with respect to the genetic make-up of primary ECs, established immortal EC lines are extensively used for studying endothelial biology. To address this issue, the immortal endothelial cell line EA.hy926 was developed by fusing primary human umbilical vein endothelial cells (HUVECs) with human lung carcinoma A549 cells. EA.hy926 cells share a number of similar endothelial properties with HUVECs and are considered the immortal counterpart to primary HUVECs. However, the cytogenetic integrity of EA.hy926 cells is not fully elucidated. We characterized EA.hy926 cells with conventional G-banding and molecular cytogenetic techniques such as spectral karyotyping and subtelomeric fluorescence in situ hybridization. Cytogenetic analysis revealed an array of numerical and stable structural chromosomal rearrangements including one deletion, one duplication, one isochromosome, seven simple translocations, and five complex translocations in Ea.hy926 cells. These findings will advance comprehension of EA.hy926 cell biology and augment future endothelial studies, specifically in comparison studies between HUVECs and EA.hy926 cells.
摘要：
内皮细胞（ECs）维持血管张力和屏障完整性，调节血液稳态，在正常生理条件下防止白细胞外渗。由于寿命有限，并且在主要EC的遗传组成方面存在批次差异，已建立的不朽EC系广泛用于研究内皮生物学。为了解决这个问题，永生内皮细胞系EA。hy926是通过将原代人脐静脉内皮细胞（HUVECs）与人肺癌A549细胞融合而开发的。EA.hy926细胞与HUVEC具有许多相似的内皮特性，被认为是原代HUVEC的永生对应物。然而,EA的细胞遗传学完整性。hy926细胞尚未完全阐明。我们表征了EA。hy926细胞具有常规G显带和分子细胞遗传学技术，例如光谱核型分析和亚端粒荧光原位杂交。细胞遗传学分析显示了一系列数量和稳定的结构染色体重排，包括一个缺失，一次重复，一个等值染色体,七个简单的易位，和Ea中的五个复杂易位。hy926细胞。这些发现将促进对EA的理解。hy926细胞生物学和加强未来的内皮研究，特别是在HUVECs和EA之间的比较研究中。hy926细胞。
