PDF(Pubmed)
Abstract：
Bio-stimulants, such as selenium nanoparticles and melatonin, regulate melon growth. However, the effects of individual and combined applications of selenium nanoparticles and melatonin on the growth of melon seedlings have not been reported. Here, two melon cultivars were sprayed with selenium nanoparticles, melatonin, and a combined treatment, and physiological and biochemical properties were analyzed. The independent applications of selenium nanoparticles, melatonin, and their combination had no significant effects on the plant heights and stem diameters of Jiashi and Huangmengcui melons. Compared with the controls, both selenium nanoparticle and melatonin treatments increased soluble sugars (6-63%) and sucrose (11-88%) levels, as well as the activity of sucrose phosphate synthase (171-237%) in melon leaves. The phenylalanine ammonia lyase (29-95%), trans cinnamate 4-hydroxylase (32-100%), and 4-coumaric acid CoA ligase (26-113%), as well as mRNA levels, also increased in the phenylpropanoid metabolism pathway. Combining the selenium nanoparticles and melatonin was more effective than either of the single treatments. In addition, the levels of superoxide dismutase (43-130%), catalase (14-43%), ascorbate peroxidase (44-79%), peroxidase (25-149%), and mRNA in melon leaves treated with combined selenium nanoparticles and melatonin were higher than in controls. The results contribute to our understanding of selenium nanoparticles and melatonin as bio-stimulants that improve the melon seedlings\' growth by regulating carbohydrate, polyamine, and antioxidant capacities.
摘要：
生物兴奋剂,例如纳米硒和褪黑激素，调节甜瓜的生长。然而,硒纳米颗粒和褪黑素单独和联合应用对甜瓜幼苗生长的影响尚未见报道。这里,对两个甜瓜品种进行了纳米硒喷雾，褪黑激素,和联合治疗，并对生理生化特性进行了分析。硒纳米粒子的独立应用，褪黑激素,它们的组合对伽师和黄蒙翠瓜的株高和茎直径没有显着影响。与对照组相比，硒纳米颗粒和褪黑激素处理都增加了可溶性糖（6-63％）和蔗糖（11-88％）水平，以及甜瓜叶片中蔗糖磷酸合酶的活性（171-237％）。苯丙氨酸氨裂解酶（29-95％），反式肉桂酸4-羟化酶（32-100％），和4-香豆酸CoA连接酶（26-113%），以及mRNA水平，苯丙素代谢途径也增加。硒纳米颗粒和褪黑激素的组合比任一单一治疗更有效。此外,超氧化物歧化酶的水平（43-130%），过氧化氢酶（14-43%），抗坏血酸过氧化物酶（44-79%），过氧化物酶（25-149%），联合硒纳米颗粒和褪黑激素处理的甜瓜叶片中的mRNA含量高于对照组。结果有助于我们了解纳米硒和褪黑激素作为生物刺激剂，通过调节碳水化合物来改善甜瓜幼苗的生长，多胺,和抗氧化能力。
