PDF(Pubmed)
Abstract：
Jinhu groupers, the hybrid offspring of tiger groupers (Epinephelus fuscoguttatus) and potato groupers (Epinephelus tukula), have excellent heterosis in fast growth and strong stress resistance. However, compared with the maternal tiger grouper, Jinhu groupers show delayed gonadal development. To explore the interspecific difference in gonadal development, we compared the transcriptomes of brain, pituitary, and gonadal tissues between Jinhu groupers and tiger groupers at 24-months old. In total, 3034 differentially expressed genes (DEGs) were obtained. KEGG (Kyoto Encyclopedia of Genes and Genomes) enrichment analyses showed that the osteoclast differentiation, oocyte meiosis, and ovarian steroidogenesis may be involved in the difference in gonadal development. Trend analysis showed that the DEGs were mainly related to signal transduction and cell growth and death. Additionally, differences in expression levels of nr4a1, pgr, dmrta2, tbx19, and cyp19a1 may be related to gonadal retardation in Jinhu groupers. A weighted gene co-expression network analysis revealed three modules (i.e., saddlebrown, paleturquoise, and greenyellow) that were significantly related to gonadal development in the brain, pituitary, and gonadal tissues, respectively, of Jinhu groupers and tiger groupers. Network diagrams of the target modules were constructed and the respective hub genes were determined (i.e., cdh6, col18a1, and hat1). This study provides additional insight into the molecular mechanism underlying ovarian stunting in grouper hybrids.
摘要：
金湖石斑鱼,老虎石斑鱼（Epinephelusfuscoguttatus）和马铃薯石斑鱼（Epinephelustukula）的杂交后代，具有优良的杂种优势，生长快，抗逆性强。然而,与母老虎石斑鱼相比，金湖石斑鱼性腺发育延迟。探讨性腺发育的种间差异,我们比较了大脑的转录组，垂体,和24个月大的金湖石斑鱼和老虎石斑鱼之间的性腺组织。总的来说,获得了3034个差异表达基因(DEGs)。KEGG（京都基因和基因组百科全书）富集分析表明，破骨细胞分化，卵母细胞减数分裂,卵巢类固醇生成可能与性腺发育的差异有关。趋势分析表明，DEGs主要与信号转导和细胞生长死亡有关。此外,nr4a1，pgr的表达水平差异，dmrta2、tbx19和cyp19a1可能与金湖石斑鱼性腺发育迟缓有关。加权基因共表达网络分析揭示了三个模块(即，马鞍棕色,Paleturquoise,和绿色）与大脑中的性腺发育显着相关，垂体,和性腺组织，分别,金湖石斑鱼和老虎石斑鱼。构建了目标模块的网络图，并确定了各自的集线器基因（即，cdh6、col18a1和hat1)。这项研究为石斑鱼杂种卵巢发育迟缓的分子机制提供了更多的见解。
