PDF(Pubmed)
Abstract：
Biomarkers, including proteins, nucleic acids, antibodies, and peptides, are essential for identifying diseases such as cancer and differentiating between healthy and abnormal cells in patients. To date, studies have shown that cancer stem cells have DNA repair mechanisms that deter the effects of medicinal treatment. Experiments with cell cultures and chemotherapy treatments of these cultures have revealed the presence of small cells, with a small amount of cytoplasm that can be intensively stained with azure eosin, called microcells. Microcells develop during sporosis from a damaged tumor macrocell. After anticancer therapy in tumor cells, a defective macrocell may produce one or more microcells. This study aims to characterize microcell morphology in melanoma cell lines. In this investigation, we characterized the population of cancer cell microcells after applying paclitaxel treatment to a Sk-Mel-28 melanoma cell line using immunocytochemical cell marker detection and fluorescent microscopy. Paclitaxel-treated cancer cells show stronger expression of stem-associated ALDH2, SOX2, and Nanog markers than untreated cells. The proliferation of nuclear antigens in cells and the synthesis of RNA in microcells indicate cell self-defense, promoting resistance to applied therapy. These findings improve our understanding of microcell behavior in melanoma, potentially informing future strategies to counteract drug resistance in cancer treatment.
摘要：
生物标志物,包括蛋白质，核酸,抗体，和肽，对于识别癌症等疾病以及区分患者的健康细胞和异常细胞至关重要。迄今为止,研究表明，癌症干细胞具有阻止药物治疗效果的DNA修复机制。细胞培养和这些培养物的化学疗法的实验揭示了小细胞的存在，少量的细胞质可以被天蓝色的伊红强烈染色，称为微细胞。在孢子病期间，微细胞从受损的肿瘤大细胞发展而来。在肿瘤细胞中进行抗癌治疗后，有缺陷的宏单元可以产生一个或多个微单元。这项研究旨在表征黑色素瘤细胞系中的微细胞形态。在这次调查中,我们使用免疫细胞化学细胞标记物检测和荧光显微镜对Sk-Mel-28黑色素瘤细胞系应用紫杉醇处理后的癌细胞微细胞群体进行了表征.与未处理的细胞相比,紫杉醇处理的癌细胞显示出更强的干相关ALDH2、S0X2和Nanog标志物的表达。细胞中核抗原的增殖和微细胞中RNA的合成表明细胞的自我防御，促进对应用治疗的抵抗。这些发现提高了我们对黑色素瘤中微小细胞行为的理解，可能为未来的策略提供信息，以抵消癌症治疗中的耐药性。
