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Abstract：
Phormidesmis communis strain AB_11_10 was isolated and identified using microscopy and 16s rRNA sequencing, and its phytochemical constituents were determined using liquid chromatography-quadrupole time-of-flight mass spectrometry. The isolate had a segmented filamentous shape with a blue-green color. Many biomolecules, including organic compounds, amino acids, and fatty acids, were detected. P. communis strain AB_11_10 was used to synthesize gold nanoparticles (Ph-AuNPs) by adjusting the optimum reaction conditions. The concentration, algal/precursor ratio, temperature, reaction time, and pH significantly influenced the synthesis of the Ph-AuNPs. Mixing 1 mL of 0.5 mM of HAuCl4 with 1 mL of algal extract and exposing the mixture to 100 °C for 30 min at pH 5.6 were the optimum conditions for the biosynthesis of Ph-AuNPs at a wavelength of 524.5 nm. The Ph-AuNPs were characterized using TEM, SEM, EDX, and mapping Zeta sizer and FTIR. The Ph-AuNPs had quasi-spherical to triangular shapes with an average diameter of 9.6 ± 4.3 nm. Ph-AuNPs composed of 76.10 ± 3.14% of Au and trace amounts of carbon and oxygen were detected, indicating that the P. communis strain AB_11_10 successfully synthesized Ph-AuNPs. The hydrodynamic diameter of the Ph-AuNPs was 28.5 nm, and their potential charge was -17.7 mV. O-H, N-H, C=C, N-O, C-H, and C-O were coated onto the surfaces of the Ph-AuNPs. These groups correspond to algal phytochemicals, which may have been the main reducing and stabilizing substances during the Ph-AuNP synthesis. The therapeutic activity of the Ph-AuNPs against osteosarcoma cancers was examined in MG-63 and SAOS-2 cell lines, while their biocompatibility was tested against Vero cell lines using a sulforhodamine B assay. The Ph-AuNPs had potent antitumor activity against the MG-63 and SAOS-2 cells, with a low toxicity toward Vero cells. Flow cytometry and cell cycle arrest analyses revealed that the Ph-AuNPs enhanced the apoptotic pathway and arrested the cell cycle in the MG-63 and SAOS-2 cells. P. communis strain AB_11_10 provides a new source to synthesize small, stable, and biocompatible AuNPs that act as apoptotic enhancers in osteosarcoma.
摘要：
分离并使用显微镜和16srRNA测序鉴定phormidesmiscommunis菌株AB_11_10，并使用液相色谱-四极杆飞行时间质谱法测定其植物化学成分。分离物具有带有蓝绿色的分段丝状形状。许多生物分子，包括有机化合物,氨基酸,和脂肪酸，被检测到。通过调整最佳反应条件，使用P.communis菌株AB_11_10合成金纳米颗粒（Ph-AuNPs）。浓度，藻类/前体比例，温度,反应时间,和pH显著影响Ph-AuNP的合成。将1mL0.5mMHAuCl4与1mL藻类提取物混合，并将混合物在pH5.6下暴露于100°C30分钟，是在524.5nm波长下生物合成Ph-AuNP的最佳条件。Ph-AuNP用TEM表征，SEM,EDX,并绘制Zetasizer和FTIR图。Ph-AuNP具有平均直径为9.6±4.3nm的准球形至三角形。检测到由76.10±3.14％的Au和痕量的碳和氧组成的Ph-AuNPs，表明P.communis菌株AB_11_10成功合成了Ph-AuNPs。Ph-AuNP的流体动力学直径为28.5nm，它们的潜在电荷为-17.7mV。O-H,N-H,C=C,N-O,C-H,和C-O被涂覆到Ph-AuNP的表面上。这些基团对应于藻类植物化学物质，在Ph-AuNP合成过程中可能是主要的还原和稳定物质。在MG-63和SAOS-2细胞系中检查了Ph-AuNPs对骨肉瘤的治疗活性，同时使用磺基罗丹明B测定法对Vero细胞系进行了生物相容性测试。Ph-AuNP对MG-63和SAOS-2细胞具有有效的抗肿瘤活性，对Vero细胞具有低毒性。流式细胞术和细胞周期停滞分析显示，Ph-AuNP增强了MG-63和SAOS-2细胞的凋亡途径并阻滞了细胞周期。P.communis菌株AB_11_10提供了合成小的新来源，稳定,和生物相容性AuNP在骨肉瘤中充当凋亡促进剂。
