PDF(Pubmed)
Abstract：
Our study examines the immunoexpression patterns of Megalin, Cubilin, Caveolin-1, Gipc1 and Dab2IP in the embryonic development (E) and postnatal (P) mouse kidney, with a focus on differentiating patterns between wild-type (wt) and yotari, Dab1-/- (yot) mice. Immunofluorescence revealed raised immunoexpression of receptors Megalin and Cubilin at the ampulla/collecting ducts and convoluted tubules across all developmental stages, with the most prominent immunoexpression observed in the convoluted tubules and the parietal epithelium of the Bowman\'s capsule. Quantitative analysis showed a higher percentage of Megalin and Cubilin in wt compared to yot mice at E13.5. Co-expression of Megalin and Cubilin was observed at the apical membrane of convoluted tubules and the parietal layer of the Bowman\'s capsule. The staining intensity of Megalin varied across developmental stages, with the strongest reactivity observed at the ampulla and collecting ducts at embryonic day (E) 13.5 in wt mice. In contrast, Caveolin-1 exhibited high immunoexpression in the metanephric mesenchyme, blood vessels, and the border area between the metanephric mesenchyme and renal vesicle, with a decrease in immunoexpression as development progressed. Gipc1 showed diffuse cytoplasmic staining in metanephric mesenchyme, convoluted tubules and collecting ducts, with significant differences in immunoexpression between wild-type and yot mice at both investigated embryonic time points. Dab2IP immunofluorescent staining was most prominent in renal vesicle/glomeruli and ampulla/collecting ducts at E13.5, with mild staining intensity observed in the distal convoluted tubules postnatally. Our findings elucidate distinct immunoexpression of patterns and potential parts of these proteins in the development and function of the kidney, highlighting the importance of further investigation into their regulatory mechanisms.
摘要：
我们的研究检查了巨蛋白的免疫表达模式，Cubilin,小窝蛋白-1、Gibc1和Dab2IP在胚胎发育（E）和出生后（P）小鼠肾脏中，重点是区分野生型（wt）和Yotari之间的模式，Dab1-/-(yot)小鼠。免疫荧光显示，在所有发育阶段，壶腹/收集管和曲小管中受体Megalin和Cubilin的免疫表达均升高。在Bowman囊的回旋小管和顶叶上皮中观察到最突出的免疫表达。定量分析显示，与E13.5小鼠相比，重量中Megalin和Cubilin的百分比更高。在回旋小管的顶膜和Bowman囊的顶层观察到Megalin和Cubilin的共表达。Megalin的染色强度随发育阶段而变化，在wt小鼠中，在胚胎天（E）13.5在壶腹和收集管处观察到最强的反应性。相比之下,小窝蛋白-1在后肾间质中表现出高免疫表达，血管,后肾间质和肾囊泡之间的边界区域，随着发育的进展，免疫表达降低。Gibc1显示后肾间质弥漫性细胞质染色，复杂的小管和收集管，在两个研究的胚胎时间点，野生型和yot小鼠之间的免疫表达存在显着差异。Dab2IP免疫荧光染色在E13.5的肾囊泡/肾小球和壶腹/收集管中最为明显，在出生后的远曲小管中观察到轻度染色强度。我们的发现阐明了这些蛋白质在肾脏发育和功能中的模式和潜在部分的独特免疫表达，强调进一步调查其监管机制的重要性。
