PDF(Pubmed)
Abstract：
A multi-strain yeast-based paraprobiotic (MsYbP) comprising inactive cells and polysaccharides (β-glucan, mannan oligosaccharides, and oligosaccharides) derived from Saccharomyces cerevisiae and Cyberlindnera jadinii could ensure optimal growth and health in farmed fish. This study assessed the impact of an MsYbP on the growth, immune responses, antioxidant capacities, and liver health of largemouth bass (Micropterus salmoides) through lab-scale (65 days) and pilot-scale (15 weeks) experiments. Two groups of fish were monitored: one fed a control diet without the MsYbP and another fed 0.08% and 0.1% MsYbP in the lab-scale and pilot-scale studies, respectively (referred to as YANG). In the lab-scale study, four replicates were conducted, with 20 fish per replicate (average initial body weight = 31.0 ± 0.8 g), while the pilot-scale study involved three replicates with approximately 1500 fish per replicate (average initial body weight = 80.0 ± 2.2 g). The results indicate that the MsYbP-fed fish exhibited a significant increase in growth in both studies (p < 0.05). Additionally, the dietary MsYbP led to a noteworthy reduction in the liver function parameters (p < 0.05), such as alanine aminotransferase (ALT), aspartate aminotransferase (AST) and alkaline phosphatase (AKP), and hepatic nuclear density, indicating improved liver health. Furthermore, the dietary MsYbP elevated the antioxidative capacity of the fish by reducing their malondialdehyde levels and increasing their levels and gene expressions related to antioxidative markers, such as total antioxidant ca-pacity (T-AOC), total superoxide dismutase (T-SOD), glutathione peroxidase (GSH-Px), catalase (CAT), nuclear factor erythroid 2-related factor 2 (nrf2) and kelch-1ike ech-associated protein (keap1) in both studies (p < 0.05). In terms of hepatic immune responses, the lab-scale study showed an increase in inflammation-related gene expressions, such as interleukin-1β (il-1β) and transforming growth factor β1 (tgf-β1), while the pilot-scale study significantly suppressed the expressions of genes related to inflammatory responses, such as tumor necrosis factor α (tnfα) and interleukin-10 (il-10) (p < 0.05). In summary, our findings underscore the role of dietary multi-strain yeast-based paraprobiotics in enhancing the growth and liver health of largemouth bass, potentially through increased antioxidative capacity and the modulation of immune responses, emphasizing the significance of employing yeast-based paraprobiotics in commercial conditions.
摘要：
一种多株基于酵母的副益生菌（MsYbP），包含非活性细胞和多糖（β-葡聚糖，甘露寡糖，和寡糖）源自酿酒酵母和Cyberlindnerajadinii可以确保养殖鱼类的最佳生长和健康。这项研究评估了MsYbP对生长的影响，免疫反应，抗氧化能力，通过实验室规模（65天）和中试规模（15周）实验，大口鲈鱼（Micropterussalmoides）的肝脏健康。监测了两组鱼：一组饲喂不含MsYbP的对照饮食，另一组在实验室规模和中试规模的研究中饲喂0.08％和0.1％MsYbP，（分别称为杨）。在实验室规模的研究中，进行了四次重复，每个重复20条鱼（平均初始体重=31.0±0.8g），而中试规模的研究涉及三个重复，每个重复约1500条鱼（平均初始体重=80.0±2.2g）。结果表明,MsYbP饲喂的鱼在两项研究中都表现出生长的显著增加(p<0.05)。此外,饮食MsYbP导致肝功能参数显着降低（p<0.05），如丙氨酸氨基转移酶（ALT），天冬氨酸转氨酶（AST）和碱性磷酸酶（AKP），和肝核密度，表明改善肝脏健康。此外，饮食MsYbP通过降低鱼类丙二醛水平，增加其水平和与抗氧化标志物相关的基因表达来提高鱼类的抗氧化能力，如总抗氧化剂容量(T-AOC)，总超氧化物歧化酶（T-SOD），谷胱甘肽过氧化物酶（GSH-Px），过氧化氢酶(CAT),在两项研究中，核因子红系2相关因子2（nrf2）和kelch-1ikeech相关蛋白（keap1）（p<0.05）。在肝脏免疫反应方面，实验室规模的研究表明，炎症相关基因表达增加，如白细胞介素-1β（il-1β）和转化生长因子β1（tgf-β1），虽然试点规模研究显著抑制了炎症反应相关基因的表达，如肿瘤坏死因子α(tnfα)和白细胞介素-10(il-10)(p<0.05)。总之,我们的发现强调了饮食多株酵母为基础的副益生菌在提高大口鲈鱼的生长和肝脏健康的作用，可能通过增加抗氧化能力和调节免疫反应，强调在商业条件下使用基于酵母的副益生菌的重要性。
