PDF(Pubmed)
Abstract：
Regenerative endodontics (REP) is a new clinical modality aiming to regenerate damaged soft and hard dental tissues, allowing for root completion in young adults\' teeth. Effective disinfection is crucial for REP success, but commonly used antimicrobials often harm the niche dental pulp stem cells (DPSCs). To our knowledge, this is the first study to explore the biocompatibility and antimicrobial potential of pectin as a potential natural intracanal medicament for REPs. Low methoxyl commercial citrus pectin (LM) (pectin CU701, Herbstreith&Fox.de) was used in all experiments. The pectin\'s antibacterial activity against single species biofilms (E. faecalis and F. nucleatum) was assessed using growth curves. The pectin\'s antimicrobial effect against mature dual-species biofilm was also evaluated using confocal laser scanning microscopy (CLSM) after 30 min and 7 days of treatment. The DPSC biocompatibility with 2% and 4% w/v of the pectin coatings was evaluated using live/dead staining, LDH, and WST-1 assays. Pectin showed a concentration-dependent inhibitory effect against single-species biofilms (E. faecalis and F. nucleatum) but failed to disrupt dual-species biofilm. Pectin at 2% w/v concentration proved to be biocompatible with the HDPSCs. However, 4% w/v pectin reduced both the viability and proliferation of the DPSCs. Low concentration (2% w/v) pectin was biocompatible with the DPSCs and showed an antimicrobial effect against single-species biofilms. This suggests the potential for using pectin as an injectable hydrogel for clinical applications in regenerative endodontics.
摘要：
再生牙髓药（REP）是一种新的临床模式，旨在再生受损的软硬牙齿组织，允许年轻人的牙齿完成牙根。有效的消毒对于REP的成功至关重要，但常用的抗菌药物往往会损害小生境牙髓干细胞(DPSC)。据我们所知,这是首次探索果胶作为REPs潜在的天然肛门内药物的生物相容性和抗菌潜力的研究。低甲氧基商品柑橘果胶（LM）（果胶CU701，Herbstreith和福克斯。de)用于所有实验。果胶对单一物种生物膜的抗菌活性(E.粪便和F.核仁）使用生长曲线进行评估。在处理30分钟和7天后，还使用共聚焦激光扫描显微镜（CLSM）评估了果胶对成熟双物种生物膜的抗微生物作用。使用活/死染色评估具有2%和4%w/v果胶涂层的DPSC生物相容性。LDH,和WST-1测定。果胶对单物种生物膜显示出浓度依赖性抑制作用（E.粪便和核仁F.），但未能破坏双物种生物膜。2%w/v浓度的果胶被证明与HDPSC生物相容。然而,4%w/v果胶降低DPSC的活力和增殖。低浓度(2%w/v)果胶与DPSC是生物相容的,并且显示针对单物种生物膜的抗微生物作用。这表明使用果胶作为可注射水凝胶用于再生牙髓的临床应用的潜力。
