PDF(Pubmed)
Abstract：
Approximately 20% of patients with colorectal cancer (CRC) are diagnosed with a mucinous subtype of this tumor, have a worse prognosis, and often show resistance to available therapies. Molecules from the mucin family are involved in the regulation of epithelial-mesenchymal transition (EMT), which significantly determines the cancer aggressiveness. This study aimed to examine the diagnostic and prognostic significance of mucinous histology and EMT markers in patients with early-onset CRC and their association with disease severity and tumor characteristics. This study included tumor tissue samples from 106 patients diagnosed with CRC before the age of 45, 53 with mucinous and 53 with non-mucinous tumors. The EMT status was determined by immunohistochemical analysis of E-cadherin and Vimentin in tissue sections. Mucinous tumors had significantly higher Mucin-1 (p < 0.001) and cytoplasmic E-cadherin (p = 0.043) scores; they were significantly less differentiated (p = 0.007), more advanced (p = 0.027), and predominately affected right the colon (p = 0.039) compared to non-mucinous tumors. Epithelial tumors were significantly better differentiated (p = 0.034) and with less prominent tumor budding (p < 0.001) than mesenchymal tumors. Mucin-1 and Vimentin were independent predictors of tumor differentiation (p = 0.006) and budding (p = 0.001), respectively. Mucinous histology and EMT markers are significant predictors of disease severity and tumor characteristics in early-onset colorectal cancer.
摘要：
大约20％的结直肠癌（CRC）患者被诊断为该肿瘤的粘液性亚型，预后较差，并经常表现出对现有疗法的抵抗力。来自粘蛋白家族的分子参与上皮-间质转化（EMT）的调节，这显著决定了癌症的侵袭性。本研究旨在探讨早发性CRC患者粘液性组织学和EMT标志物的诊断和预后意义及其与疾病严重程度和肿瘤特征的关系。该研究包括来自106例45岁之前诊断为CRC的患者的肿瘤组织样本,其中53例为粘液性肿瘤,53例为非粘液性肿瘤。通过组织切片中E-钙黏着蛋白和波形蛋白的免疫组织化学分析确定EMT状态。粘液性肿瘤的粘蛋白-1（p<0.001）和胞质E-cadherin（p=0.043）评分明显较高；它们的分化程度明显较低（p=0.007），更先进（p=0.027），与非粘液性肿瘤相比，主要影响右侧结肠（p=0.039）。与间质肿瘤相比，上皮肿瘤的分化明显更好（p=0.034），并且肿瘤出芽较少（p<0.001）。粘蛋白-1和波形蛋白是肿瘤分化(p=0.006)和出芽(p=0.001)的独立预测因子。分别。黏液组织学和EMT标志物是早发性结直肠癌疾病严重程度和肿瘤特征的重要预测因子。
