Abstract：
To better enhance printing effects meanwhile casting functionality, antioxidation and absorption of bioactive component in printed Ca2+-nano starch (NS)-lutein (L)-surimi were investigated. Results shown that Ca2+-NS-L promoted surimi printability due to enhanced gel strength and denser structure. Mixing Ca2+-NS-L endowed printed surimi with antioxidation (DPPH, ABTS, hydroxyl radical, Fe2+ reduction were 42 %, 79 %, 65 %, 0.104 mg·mL-1, respectively) due to the ability of lutein with more -OH groups and conjugate bonds to capture free radicals. It also manifested in cellular antioxidation that Ca2+-NS-L-surimi regulated the level of Nrf2 to protect gene expression of antioxidases (SOD, CAT, GSH-Px increased by 30-180 %, compared to damaged cells) through keap1-Nrf2-ARE pathway. Additionally, lutein absorption and transportation of Ca2+-NS-L-surimi increased by 20 %, compared to NS-L. Possibly, combination of samples and membrane was facilitated by surface hydrophobic, promoting endocytosis. Meanwhile, digestive surimi (peptides) with acidic-alkaline amino acids and negative charges made samples be attracted and moved in bypass parts under electrostatic traction and repulsion (electrostatic domain) to promote transport process. Also, Ca2+ facilitated CaM expression in membrane and formed Ca2+ channel by combining with CaM to accelerate entry of samples into cells. Conclusively, Ca2+-NS-L both strengthened printability of surimi and antioxidation, promoting application of printed functional surimi.
摘要：
为了更好地增强打印效果，同时铸造功能，研究了印刷Ca2-纳米淀粉（NS）-叶黄素（L）-鱼糜中生物活性成分的抗氧化和吸收。结果表明，Ca2-NS-L由于增强的凝胶强度和更致密的结构而提高了鱼糜的可印刷性。混合Ca2+-NS-L赋予印花鱼糜抗氧化(DPPH，ABTS,羟基自由基，Fe2+减少了42%,79%,65%,分别为0.104mg·mL-1），这是由于具有更多-OH基团和共轭键的叶黄素捕获自由基的能力。在细胞抗氧化作用中还表现为Ca2-NS-L-鱼糜调节Nrf2的水平以保护抗氧化酶的基因表达（SOD，CAT,GSH-Px增加了30-180%，与受损细胞相比）通过keap1-Nrf2-ARE途径。此外,叶黄素对Ca2+-NS-L-鱼糜的吸收和转运增加了20%,与NS-L相比可能,表面疏水促进了样品和膜的结合，促进内吞作用。同时,具有酸性-碱性氨基酸和负电荷的消化鱼糜（肽）使样品在静电牵引和排斥（静电域）下被吸引并在旁路部分移动，以促进运输过程。此外，Ca2+促进CaM在膜中的表达,并通过与CaM结合以加速样品进入细胞而形成Ca2+通道。最后，Ca2+-NS-L增强鱼糜的适印性和抗氧化能力,促进印花功能性鱼糜的应用。
