Abstract：
The oral cavity provides an ideal environment for microorganisms, including bacteria, viruses, and fungi, to flourish. Increasing attention has been focused on the connection between the oral microbiome and both oral and systemic diseases, spurring active research into the collection and analysis of specimens for healthcare purposes. Among the various methods for analyzing the oral microbiome, saliva analysis is especially prominent. Saliva samples, which can be collected non-invasively, provide information on the systemic health and oral microbiome composition of an individual. This review was performed to evaluate the current state of the relevant research through an examination of the literature and to suggest an appropriate assay method for investigating the oral microbiome. We analyzed articles published in English in SCI(E) journals after January 1, 2000, ultimately selecting 53 articles for review. Articles were identified through keyword searches in the PubMed, Embase, Cochrane, Web of Science, and CINAHL databases. Three experienced researchers conducted full-text assessments following title and abstract screening to select appropriate papers. Subsequently, they organized and analyzed the desired data. Our review revealed that most studies utilized unstimulated saliva samples for oral microbiome analysis. Of the 53 studies examined, 29 identified relationships between the oral microbiome and various diseases, such as oral disease, Behçet disease, cancer, and oral lichen planus. However, the studies employed diverse methods of collection and analysis, which compromised the reliability and accuracy of the findings. To address the limitations caused by methodological inconsistencies, a standardized saliva assay should be established.
摘要：
口腔为微生物提供了理想的环境，包括细菌,病毒,和真菌，蓬勃发展。越来越多的注意力集中在口腔微生物组与口腔和全身性疾病之间的联系上。促进积极的研究，以收集和分析标本为目的的医疗保健。在分析口腔微生物组的各种方法中，唾液分析尤其突出。唾液样本,可以非侵入地收集，提供有关个体的全身健康和口腔微生物组组成的信息。进行这篇综述是为了通过审查文献来评估相关研究的当前状态，并提出一种用于调查口腔微生物组的适当测定方法。我们分析了2000年1月1日之后在SCI（E）期刊上发表的英文文章，最终选择了53篇文章进行审查。文章是通过PubMed中的关键字搜索来识别的，Embase,科克伦,WebofScience,和CINAHL数据库。三位经验丰富的研究人员在标题和摘要筛选后进行了全文评估，以选择合适的论文。随后，他们组织并分析了所需的数据。我们的综述显示，大多数研究利用未刺激的唾液样本进行口腔微生物组分析。在检查的53项研究中，29确定口腔微生物组和各种疾病之间的关系，如口腔疾病，Behçet病,癌症,和口腔扁平苔藓.然而,这些研究采用了不同的收集和分析方法，这损害了研究结果的可靠性和准确性。为了解决方法不一致造成的局限性，应建立标准化的唾液检测方法。
