PDF(Pubmed)
Abstract：
Litsea martabanica (Kurz) Hook.f. has traditionally been used as an anti-insecticidal agent and as a medication due to its hepatoprotective properties by highland communities in Thailand. This study examined the mutagenicity, as well as the acute and chronic toxicity, of the L. martabanica water leaf extract in Sprague-Dawley rats. The pharmacognostic evaluation of L. martabanica was performed in this study to ensure its authenticity and purity. Then, the sample was extracted using decoction with water to obtain the crude water extract. The assessment of acute toxicity involved a single oral administration of 5000 mg/kg, whereas the chronic toxicity assessment comprised daily oral doses of 250, 750, and 2250 mg/kg over 270 days. Various physiological and behavioral parameters, as well as body and organ weights, were systematically monitored. The endpoint assessments involved hematological and biochemical analyses plus gross and histopathological assessments of the internal organs. Our results exhibited no mutagenic activation by the L. martabanica water leaf extract in the Ames test, and no acute toxicity was observed. In the chronic toxicity tests, no abnormalities were found in rats receiving the L. martabanica water leaf extract across multiple measures, comprising behavioral, physiological, and hematological indices. Crucially, the histopathological assessment corroborated previous studies, reporting an absence of any tissue abnormalities. The results revealed that the L. martabanica water leaf extract had no adverse effects on rats over 270 days of oral administration. This demonstrates its safety and crucial scientific evidence for informing public policy and enabling its potential future commercial use in both highland and lowland communities.
摘要：
Litseamartabanica（Kurz）Hook.f。由于泰国高地社区的保肝特性，传统上一直被用作抗杀虫剂和药物。这项研究检查了诱变性，以及急性和慢性毒性，在Sprague-Dawley大鼠中的马tabanica水叶提取物。在这项研究中进行了马氏乳杆菌的生药学评估，以确保其真实性和纯度。然后，用水煎剂提取样品，得到粗水提取物。急性毒性的评估涉及5000mg/kg的单次口服给药，而慢性毒性评估包括270天内每日口服剂量250,750和2250mg/kg.各种生理和行为参数，以及身体和器官的重量，系统监测。终点评估涉及血液学和生化分析以及内部器官的总体和组织病理学评估。我们的结果表明，在Ames试验中，马塔巴尼卡L.martabanica水叶提取物没有诱变活化，未观察到急性毒性。在慢性毒性试验中,在接受马氏乳杆菌水叶提取物的大鼠中，通过多种措施均未发现异常，包括行为，生理,和血液学指标。至关重要的是，组织病理学评估证实了以前的研究，报告没有任何组织异常。结果表明，口服给药270天对大鼠无不良影响。这证明了其安全性和重要的科学证据，可以为公共政策提供信息，并使其在高地和低地社区的潜在未来商业使用成为可能。
