PDF(Pubmed)
Abstract：
Septic arthritis (SA) in horses has long-term health implications. The success of its resolution hinges on the implementation of early, aggressive treatment, which is often sustained over a prolonged period. Common diagnostic methods do not allow for the reliable detection of the eradication of joint infection. A potential alternative is the discovery and characterization of mRNA biomarkers. The purpose of this study was to identify potential mRNA biomarkers for the eradication of joint infection in equine SA and to compare their expression with our previously published proteomics data. In addition, the transcriptomics data were compared to the mRNA biomarker panel, SeptiCyte Lab, used to distinguish sepsis from non-septic shock in humans. A comparative transcriptomics analysis of synovial fluid from the SA joints of five horses with active infection and subsequent post-treatment eradicated infection in the same joints and five horses with non-septic synovitis was performed. Eight novel mRNA transcripts were identified that were significantly upregulated (>3-fold) in horses with active SA compared to horses post-eradication of infection after treatment and horses with non-septic synovitis. Two proteins in our proteomics data corresponded to these mRNA transcripts, but were not statistically different. The transcripts used in the SeptiCyte test were not differentially expressed in our study. Our results suggest that mRNA may be a useful source of biomarkers for the eradication of joint infection in horses and warrants further investigation.
摘要：
马的化脓性关节炎（SA）具有长期的健康影响。其决议的成功取决于尽早实施，积极治疗,这通常是长期持续的。常见的诊断方法不能可靠地检测关节感染的根除。潜在的替代方案是mRNA生物标志物的发现和表征。这项研究的目的是确定根除马SA中关节感染的潜在mRNA生物标志物，并将其表达与我们先前发表的蛋白质组学数据进行比较。此外,将转录组学数据与mRNA生物标志物组进行比较，SeptiCyte实验室,用于区分人类败血症和非败血症性休克。对五匹患有活动性感染的马的SA关节的滑液进行了比较转录组学分析，随后在相同的关节和五匹患有非感染性滑膜炎的马的治疗后根除了感染。与治疗后根除感染后的马和患有非感染性滑膜炎的马相比,鉴定了8种新的mRNA转录本,其在具有活性SA的马中显著上调(>3倍)。我们的蛋白质组学数据中的两种蛋白质对应于这些mRNA转录本，但没有统计学差异。SeptiCyte测试中使用的转录本在我们的研究中没有差异表达。我们的结果表明，mRNA可能是根除马关节感染的有用生物标志物来源，值得进一步研究。
