PDF(Pubmed)
Abstract：
Nutrition plays an important role in shaping the gut microbiome composition, although the impact of diet on the urinary microbiome (i.e., urobiome) remains unknown. The aim of this pilot study was to discover how nutritional features affect the diversity and composition of the urobiome in dogs. Dietary histories were obtained for 15 clinically healthy adult dogs, including limited nutrient (protein, fat, crude fiber), commercial diet brand, and dietary diversity profiles. The urine samples were collected via cystocentesis, followed by sequencing of the bacterial 16S rRNA gene. The data were analyzed to determine associations between major nutrients and dietary sources with the urobiome\'s composition. The protein, fat, and crude fiber contents had no statistically significant effect on the alpha or beta diversity. However, the beta diversity values differed (PERMANOVA; p = 0.017, R2 = 0.10) between dogs fed one commercial diet brand compared to dogs consuming any other brand. The beta diversity values also differed (p = 0.019, R2 = 0.10) between dogs consuming more diverse daily diets compared to those consuming less diverse diets (≥3 or <3 unique food sources, respectively). Overall, the results of this pilot study suggest that diet might impact the urobiome and support further exploration of the relationship between diet and the urobiome\'s composition in dogs.
摘要：
营养在塑造肠道微生物组组成中起着重要作用，尽管饮食对尿液微生物组的影响(即，urobiome）仍然未知。这项初步研究的目的是发现营养特征如何影响狗的尿路生物群的多样性和组成。获得了15只临床健康成年犬的饮食史，包括有限的营养素(蛋白质，脂肪，粗纤维)，商业饮食品牌，和饮食多样性概况。通过膀胱穿刺术收集尿液样本，然后对细菌16SrRNA基因进行测序。对数据进行了分析，以确定主要营养素和饮食来源与泌尿系组成之间的关联。蛋白质,脂肪，和粗纤维含量对α或β多样性没有统计学意义。然而,与食用任何其他品牌的狗相比，喂食一种商业饮食品牌的狗之间的β多样性值有所不同（PERMANOVA;p=0.017，R2=0.10）。β多样性值也有差异（p=0.019，R2=0.10），与那些消耗不同饮食（≥3或<3种独特食物来源，分别)。总的来说,这项初步研究的结果表明，饮食可能会影响泌尿系，并支持进一步探索饮食与狗泌尿系组成之间的关系。
