PDF(Pubmed)
Abstract：
Abrin and ricin, both type II ribosome-inactivating proteins, are toxins of significant concern and are under international restriction by the Chemical Weapons Convention and the Biological and Toxin Weapons Convention. The development of a rapid and sensitive detection method for these toxins is of the utmost importance for the first emergency response. Emerging rapid detection techniques, such as surface-enhanced Raman spectroscopy (SERS) and lateral flow assay (LFA), have garnered attention due to their high sensitivity, good selectivity, ease of operation, low cost, and disposability. In this work, we generated stable and high-affinity nanotags, via an efficient freezing method, to serve as the capture module for SERS-LFA. We then constructed a sandwich-style lateral flow test strip using a pair of glycoproteins, asialofetuin and concanavalin A, as the core affinity recognition molecules, capable of trace measurement for both abrin and ricin. The limit of detection for abrin and ricin was 0.1 and 0.3 ng/mL, respectively. This method was applied to analyze eight spiked white powder samples, one juice sample, and three actual botanic samples, aligning well with cytotoxicity assay outcomes. It demonstrated good inter-batch and intra-batch reproducibility among the test strips, and the detection could be completed within 15 min, indicating the suitability of this SERS-LFA method for the on-site rapid detection of abrin and ricin toxins.
摘要：
Abrin和蓖麻毒素,两种II型核糖体失活蛋白，受到《化学武器公约》和《生物和毒素武器公约》的国际限制。这些毒素的快速灵敏检测方法的开发对于首次应急响应至关重要。新兴的快速检测技术，如表面增强拉曼光谱（SERS）和侧流测定（LFA），由于它们的高灵敏度而引起了人们的注意，良好的选择性，操作方便,低成本,和可处置性。在这项工作中,我们产生了稳定的高亲和力纳米标签，通过有效的冷冻方法，用作SERS-LFA的捕获模块。然后，我们使用一对糖蛋白构建了三明治式的侧向流动测试条，asialofetuin和伴刀豆球蛋白A，作为核心亲和识别分子，能够对Abrin和蓖麻毒素进行痕量测量。Abrin和蓖麻毒素的检测限为0.1和0.3ng/mL，分别。该方法用于分析8个加标白色粉末样品，一份果汁样本,和三个实际的植物样本，与细胞毒性测定结果吻合良好。它在测试条之间显示出良好的批次间和批次内再现性，检测可以在15分钟内完成，表明这种SERS-LFA方法适用于现场快速检测abrin和蓖麻毒素。
