PDF(Pubmed)
Abstract：
In this study, a functional nanostructured lipid carriers (NLCs)-based hydrogel was developed to repair the damaged epidermal skin barrier. NLCs were prepared via a high-energy approach, using argan oil and beeswax as liquid and solid lipids, respectively, and were loaded with ceramides and cholesterol at a physiologically relevant ratio, acting as structural and functional compounds. Employing a series of surfactants and optimizing the preparation conditions, NLCs of 215.5 ± 0.9 nm in size and a negative zeta potential of -42.7 ± 0.9 were obtained, showing acceptable physical and microbial stability. Solid state characterization by differential scanning calorimetry and X-ray powder diffraction revealed the formation of imperfect crystal NLC-type. The optimized NLC dispersion was loaded into the gel based on sodium hyaluronate and xanthan gum. The gels obtained presented a shear thinning and thixotropic behavior, which is suitable for dermal application. Incorporating NLCs enhanced the rheological, viscoelastic, and textural properties of the gel formed while retaining the suitable spreadability required for comfortable application and patient compliance. The NLC-loaded gel presented a noticeable occlusion effect in vitro. It provided 2.8-fold higher skin hydration levels on the ex vivo porcine ear model than the NLC-free gel, showing a potential to repair the damaged epidermal barrier and nourish the skin actively.
摘要：
在这项研究中,开发了一种基于功能性纳米结构脂质载体（NLC）的水凝胶来修复受损的表皮皮肤屏障。NLC是通过高能方法制备的，使用摩洛哥坚果油和蜂蜡作为液体和固体脂质，分别,并以生理相关比例加载神经酰胺和胆固醇，作为结构和功能化合物。使用一系列表面活性剂并优化制备条件，获得215.5±0.9nm大小的NLC和-42.7±0.9的负ζ电位，表现出可接受的物理和微生物稳定性。通过差示扫描量热法和X射线粉末衍射进行的固态表征表明，形成了不完善的NLC型晶体。将优化的NLC分散体加载到基于透明质酸钠和黄原胶的凝胶中。获得的凝胶表现出剪切稀化和触变行为，适用于皮肤应用。掺入NLC增强了流变学，粘弹性,和所形成的凝胶的质地性质,同时保持舒适施用和患者依从性所需的合适铺展性。负载NLC的凝胶在体外表现出明显的闭塞效应。它在离体猪耳模型上提供了比无NLC凝胶高2.8倍的皮肤水合水平，显示出修复受损的表皮屏障和积极滋养皮肤的潜力。
