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Abstract：
The detection of cryptic species in complexes that have undergone recent speciation is often difficult, since many standard nuclear markers have not yet accumulated differences between closely related taxa, and differences in mitochondrial markers can be leveled out due to mitochondrial introgressions. In these cases, the use of derived chromosomal characters such as non-ancestral chromosomal numbers and/or unusual karyotype features may be a solution to the species delimitation problem. However, non-ancestral but similar karyotypes may arise secondarily as a result of homoplastic evolution, and their interpretation as homologies may lead to incorrect taxonomic conclusions. In our study, we show that the combined use of mitochondrial DNA barcodes and karyotypes helps to solve this problem and identifies cryptic species in situations where each of these markers does not work individually. Using this approach, we show that the fauna of Armenia and adjacent Iran includes the following cryptic taxa of the Polyommatus ripartii species complex (haploid chromosome number, n in parentheses): P. ripartii paralcestis (n = 90), P. ripartii kalashiani, subsp. nov (n close to 90), P. emmeli, sp. nov. (n = 77-79), P. keleybaricus, sp. nov. (n = 86), P. demavendi belovi (n = 73-75), P. demavendi antonius, subsp. nov. (n = 71-73), P. admetus anatoliensis (n = 79) and P. eriwanensis (n = 29-34). Polyommatus admetus yeranyani is synonymized with P. admetus anatoliensis.
摘要：
在经历了最近物种形成的复合物中检测隐蔽物种通常很困难，由于许多标准核标记尚未积累密切相关的分类单元之间的差异，由于线粒体渗入，线粒体标记的差异可以消除。在这些情况下,使用衍生的染色体特征，例如非祖先染色体数量和/或异常核型特征可能是物种划界问题的解决方案。然而,非祖先但相似的核型可能是同质进化的结果，它们作为同源性的解释可能会导致不正确的分类学结论。在我们的研究中,我们表明，线粒体DNA条形码和核型的结合使用有助于解决这个问题，并在这些标记中的每一个都不能单独起作用的情况下识别隐蔽物种。使用这种方法，我们表明，亚美尼亚和邻近伊朗的动物区系包括以下Polyommatusripartii物种复合体的隐秘分类群（单倍体染色体数，括号中的n)：P.ripartiiparalcestis(n=90)，P.ripartiikalashiani,subsp.nov（n接近90），P.Emmeli,sp.11月。（n=77-79），P.Keleybaricus,sp.11月。（n=86），P.demavendibelovi（n=73-75），P.DemavendiAntonius,subsp.11月。（n=71-73），黑曲霉(n=79)和绿藻(n=29-34)。多ommatusadmetusyeranyani与阿纳托利氏菌同义。
