PDF(Pubmed)
Abstract：
How alien pests invade new areas has always been a hot topic in invasion biology. The spread of the Bactrocera dorsalis from southern to northern China involved changes in food sources. In this paper, in controlled conditions, we take Bactrocera dorsalis as an example to study how plant host transformation affects gut bacteria by feeding it its favorite host oranges in the south, its favorite host peaches and apples in the north, and feeding it cucumbers as a non-favorite host plant, thereby further affecting their fitness during invasion. The result showed that, after three generations of feeding on cucumbers, Bactrocera dorsalis took longer to develop as a larva while its longevity and fecundity decreased and pre-adult mortality increased. Feeding it cucumbers significantly reduced the overall diversity of gut microbiota of Bactrocera dorsalis. The relative abundance of Enterobacter necessary for survival decreased, while the Empedobacter and Enterococcus increased, resulting in decreased carbohydrate transport and metabolism and increased lipid transport and metabolism. Feeding Bactrocera dorsalis Empedobacter brevis and Enterococcus faecalis resulted in a 26% increase in pre-adult mortality and a 2-3 d increase in adult preoviposition period (APOP). Additionally, Enterococcus faecalis decreased the longevity of female and male adults by 17 and 12 d, respectively, and decreased fecundity by 11%. We inferred that the shifted plant hosts played an important role in posing serious harm to Bactrocera dorsalis invading from the south to the north. Therefore, after an invasion of Bactrocera dorsalis into northern China, it is difficult to colonize cucumbers for a long time, but there is still a risk of short-term harm. The findings of this study have established that the interactions between an insect\'s food source and gut bacteria may have an important effect on insect invasions.
摘要：
外来害虫如何入侵新区域一直是入侵生物学的热门话题。从中国南部到北部的背实杆菌的传播涉及食物来源的变化。在本文中,在受控条件下，我们以背芽孢杆菌为例，研究植物宿主转化如何通过在南方饲喂其最喜欢的宿主橙来影响肠道细菌，它在北方最喜欢的寄主桃子和苹果，并将黄瓜作为不喜欢的寄主植物，从而进一步影响他们在入侵期间的健康。结果表明,在吃了三代黄瓜之后，背细菌需要更长的时间才能发育为幼虫，而其寿命和繁殖力却降低了，成年前死亡率增加了。饲喂黄瓜显着降低了背实菌肠道菌群的总体多样性。存活所必需的肠杆菌的相对丰度降低，而Empedobacter和肠球菌增加，导致碳水化合物运输和代谢减少，脂质运输和代谢增加。饲喂背杆菌短杆菌和粪肠球菌导致成年前死亡率增加26％，成年前产卵期（APOP）增加2-3d。此外,粪肠球菌使女性和男性成人的寿命降低了17和12d，分别,繁殖力下降11%。我们推断，转移的植物寄主在对从南向北入侵的背实菌构成严重危害方面发挥了重要作用。因此,在背细菌入侵中国北方之后，很难长时间定植黄瓜，但仍有短期伤害的风险。这项研究的发现已经确定，昆虫的食物来源和肠道细菌之间的相互作用可能对昆虫的入侵有重要影响。
