PDF(Pubmed)
Abstract：
Tenebrio molitor larvae represent a sustainable protein source for food and feed. The aim of this study was to evaluate the supplementation of chestnut shell, a by-product of the agro-industrial chain, in growth substrates for T. molitor larvae rearing. Seven-week-old larvae were reared on three different growth substrates: the control group (CTRL) was fed wheat bran, treatment group one was fed wheat bran supplemented with 12.5% w/w chestnut shell (TRT1), and treatment group two was fed wheat bran supplemented with 25% w/w chestnut shell (TRT2). Larval weight, substrate consumption, and mortality were recorded weekly. After 14 days, insect meals were produced for bromatological and colorimetric analysis, and bacterial inhibition activity assay using a microdilution method. The amino acid profile of insects was determined using quantitative nuclear magnetic resonance spectroscopy. Our results showed a lower feed conversion ratio and higher larval survival rate % in TRT2 compared to CTRL (p < 0.05). Proteins and lipids of TRT2 were higher than other groups (p < 0.05). Important differences were observed in the amino acid profile of TRT1 and TRT2 compared to CTRL (p < 0.05). TRT1 and TRT2 showed higher E. coli inhibitory activity than CTRL (p < 0.05). In conclusion, chestnut shell supplementation improved the survival and functional characteristics of larvae and likely impacted the insects\' metabolism.
摘要：
黄粉虫幼虫代表了食品和饲料的可持续蛋白质来源。这项研究的目的是评估板栗壳的补充，农业产业链的副产品，在毛虫幼虫饲养的生长基质中。在三种不同的生长基质上饲养了七周大的幼虫：对照组（CTRL）饲喂麦麸，处理组1饲喂小麦麸皮，补充12.5％w/w栗壳（TRT1），和处理组2饲喂小麦麸皮，补充25％w/w栗壳（TRT2）。幼虫体重,底物消耗，每周记录死亡率。14天后,昆虫粉被生产用于肉用和比色分析，和使用微量稀释法的细菌抑制活性测定。使用定量核磁共振波谱测定昆虫的氨基酸谱。我们的结果表明，与CTRL相比，TRT2的饲料转化率较低，幼虫存活率较高（p<0.05）。TRT2的蛋白质和脂质高于其他组（p<0.05）。与CTRL相比,在TRT1和TRT2的氨基酸谱中观察到重要差异(p<0.05)。TRT1和TRT2显示出比CTRL更高的大肠杆菌抑制活性(p<0.05)。总之,补充板栗壳改善了幼虫的存活和功能特性，并可能影响昆虫的新陈代谢。
