PDF(Pubmed)
Abstract：
Biological ageing refers to the gradual decrease in physiological functions, resulting in immune senescence, cellular damage and apoptosis. Telomere length is a biomarker of biological ageing. Limited studies have associated shorter telomere length with HIV and parasite single infections, with no studies reporting the association of HIV and parasite co-infection with telomere length. The study aimed to investigate whether telomere length shortening is accelerated in a South African population co-infected with HIV and helminths compared to participants singly infected with either HIV or helminths. Additionally, telomere length data were compared with participants\' biochemical and full blood count parameters. A total of 200 participants were in groups of uninfected control, HIV single infection, helminth single infection and HIV and helminth co-infection groups. Relative telomere length (RTL) was determined using Real-Time PCR and associated with biochemical and full blood count parameters using multivariate regression analysis models that were adjusted for confounders. The uninfected control group was used as a reference group. The uninfected control group had the highest mean RTL (1.21 ± 0.53) while the HIV-infected (0.96 ± 0.42) and co-infected (0.93 ± 0.41) groups had similar RTLs, and lastly, the helminth-infected group (0.83 ± 0.33) had the lowest RTL (p = 0.0002). When compared to the uninfected control group, a significant association between RTL and biochemical parameters, including blood iron (β = -0.48), ferritin (β = -0.48), transferrin saturation (β = -0.57), transferrin (β = -0.57), phosphate (β = -0.47), vitamin A (β = -0.49) and C-reactive protein (β = -0.52) were noted in the co-infected group (p < 0.05). In addition, a significant association between RTL and full blood count, including (β = -0.47), haematocrit (β = -0.46), mean corpuscular volume (β = -0.47), lymphocytes (β = -0.45), mean corpuscular haemoglobin concentration (β = -0.45), red cell distribution width (β = -0.47), monocytes (β = -0.45), eosinophils (β = -0.45), basophils (β = -0.44) and transferrin saturation (β = -0.57) were also noted in the co-infected group (p < 0.05). Accelerated biological ageing, as indicated by telomere length shortening, is associated with HIV and helminth co-infections.
摘要：
生物衰老是指生理功能逐渐下降，导致免疫衰老，细胞损伤和凋亡。端粒长度是生物衰老的生物标志物。有限的研究已经将较短的端粒长度与HIV和寄生虫单一感染相关联。没有研究报告HIV和寄生虫共同感染与端粒长度的关联。该研究旨在调查与单独感染HIV或蠕虫的参与者相比，同时感染HIV和蠕虫的南非人群端粒长度缩短是否加速。此外,将端粒长度数据与参与者的生化和全血计数参数进行比较。共有200名参与者处于未感染对照组中，艾滋病毒单一感染，蠕虫单一感染和HIV和蠕虫共感染组。使用Real-TimePCR确定相对端粒长度（RTL），并使用针对混杂因素进行调整的多元回归分析模型将其与生化和全血计数参数相关联。未感染的对照组作为参照组。未感染对照组的平均RTL最高（1.21±0.53），而HIV感染（0.96±0.42）和共感染（0.93±0.41）组的RTL相似，最后，蠕虫感染组（0.83±0.33）的RTL最低（p=0.0002）。与未感染的对照组相比，RTL和生化参数之间的显著关联，包括血铁（β=-0.48），铁蛋白（β=-0.48），转铁蛋白饱和度（β=-0.57），转铁蛋白（β=-0.57），磷酸盐（β=-0.47），在共感染组中观察到维生素A（β=-0.49）和C反应蛋白（β=-0.52）（p<0.05）。此外,RTL和全血细胞计数之间的显著关联，包括(β=-0.47)，血细胞比容（β=-0.46），平均红细胞体积（β=-0.47），淋巴细胞（β=-0.45），平均红细胞血红蛋白浓度（β=-0.45），红细胞分布宽度(β=-0.47)，单核细胞（β=-0.45），嗜酸性粒细胞（β=-0.45），嗜碱性粒细胞（β=-0.44）和转铁蛋白饱和度（β=-0.57）在共感染组中也被发现（p<0.05）。加速生物老化，如端粒长度缩短所示，与艾滋病毒和蠕虫共同感染有关。
