PDF(Pubmed)
Abstract：
The umbilical cord is a material that enhances regeneration and is devoid of age-related changes in the extracellular matrix (ECM). The aim of this work was to develop a biodegradable scaffold from a decellularized human umbilical cord (UC-scaffold) to heal full-thickness wounds. Decellularization was performed with 0.05% sodium dodecyl sulfate solution. The UC-scaffold was studied using morphological analysis methods. The composition of the UC-scaffold was studied using immunoblotting and Fourier transform infrared spectroscopy. The adhesion and proliferation of mesenchymal stromal cells were investigated using the LIVE/DEAD assay. The local reaction was determined by subcutaneous implantation in mice (n = 60). A model of a full-thickness skin wound in mice (n = 64) was used to assess the biological activity of the UC-scaffold. The proposed decellularization method showed its effectiveness in the umbilical cord, as it removed cells and retained a porous structure, type I and type IV collagen, TGF-β3, VEGF, and fibronectin in the ECM. The biodegradation of the UC-scaffold in the presence of collagenase, its stability during incubation in hyaluronidase solution, and its ability to swell by 1617 ± 120% were demonstrated. Subcutaneous scaffold implantation in mice showed gradual resorption of the product in vivo without the formation of a dense connective tissue capsule. Epithelialization of the wound occurred completely in contrast to the controls. All of these data suggest a potential for the use of the UC-scaffold.
摘要：
脐带是一种增强再生的材料，并且在细胞外基质（ECM）中没有年龄相关的变化。这项工作的目的是从脱细胞的人脐带（UC-支架）中开发出一种可生物降解的支架，以治愈全层伤口。用0.05%十二烷基硫酸钠溶液进行去细胞化。使用形态学分析方法研究UC-支架。使用免疫印迹和傅立叶变换红外光谱研究了UC支架的组成。使用LIVE/DEAD测定法研究间充质基质细胞的粘附和增殖。通过皮下植入小鼠(n=60)确定局部反应。使用小鼠(n=64)的全厚度皮肤伤口模型来评估UC支架的生物活性。提出的脱细胞方法在脐带中显示了其有效性，因为它去除了细胞并保留了多孔结构，I型和IV型胶原蛋白，TGF-β3,VEGF,和ECM中的纤连蛋白。在胶原酶存在下UC支架的生物降解，在透明质酸酶溶液中孵育期间的稳定性，并证明了其膨胀1617±120%的能力。小鼠皮下支架植入显示产品在体内逐渐吸收，而没有形成致密的结缔组织囊。与对照组相比，伤口的上皮化完全发生。所有这些数据表明使用UC-支架的潜力。
