PDF(Pubmed)
Abstract：
Mesenchymal stem cells (MSCs) possess significant differentiation potential, making them highly promising in medicine and immunotherapy due to their regenerative capabilities and exosome secretion. However, challenges such as limited cell divisions and complex testing hinder large-scale MSC production. In this study, we successfully established an immortalized MSC line by transfecting the human telomerase reverse transcriptase (TERT) gene into MSCs isolated from pregnant sheep umbilical cords. This approach effectively inhibits cell senescence and promotes cell proliferation, enabling the generation of umbilical cord mesenchymal stem cells (UCMSCs) on a larger scale. Our findings demonstrate that these transfected TERT-UCMSCs exhibit enhanced proliferative capacity and a reduced aging rate compared to regular UCMSCs while maintaining their stemness without tumorigenicity concerns. Consequently, they hold great potential for medical applications requiring large quantities of functional MSCs.
摘要：
间充质干细胞(MSCs)具有显著的分化潜能,由于它们的再生能力和外泌体分泌，使它们在医学和免疫疗法中非常有前途。然而,有限的细胞分裂和复杂的测试等挑战阻碍了大规模MSC的生产。在这项研究中,通过将人端粒酶逆转录酶（TERT）基因转染到从怀孕的绵羊脐带中分离的MSC中，我们成功地建立了永生化的MSC系。这种方法有效抑制细胞衰老，促进细胞增殖，脐带间充质干细胞(UCMSCs)的大规模生成。我们的发现表明，与常规UCMSC相比，这些转染的TERT-UCMSC表现出增强的增殖能力和降低的衰老速率，同时保持其干性而没有致瘤性问题。因此,它们在需要大量功能性MSC的医疗应用中具有巨大潜力。
