PDF(Pubmed)
Abstract：
A coenzyme A (CoA-SH)-responsive dual electrochemical and fluorescence-based sensor was designed utilizing an MnO2-immobilized-polymer-dot (MnO2@D-PD)-coated electrode for the sensitive detection of osteoarthritis (OA) in a peroxisomal β-oxidation knockout model. The CoA-SH-responsive MnO2@D-PD-coated electrode interacted sensitively with CoA-SH in OA chondrocytes, triggering electroconductivity and fluorescence changes due to cleavage of the MnO2 nanosheet on the electrode. The MnO2@D-PD-coated electrode can detect CoA-SH in immature articular chondrocyte primary cells, as indicated by the significant increase in resistance in the control medium (R24h = 2.17 MΩ). This sensor also sensitively monitored the increase in resistance in chondrocyte cells in the presence of acetyl-CoA inducers, such as phytol (Phy) and sodium acetate (SA), in the medium (R24h = 2.67, 3.08 MΩ, respectively), compared to that in the control medium, demonstrating the detection efficiency of the sensor towards the increase in the CoA-SH concentration. Furthermore, fluorescence recovery was observed owing to MnO2 cleavage, particularly in the Phy- and SA-supplemented media. The transcription levels of OA-related anabolic (Acan) and catabolic factors (Adamts5) in chondrocytes also confirmed the interaction between CoA-SH and the MnO2@D-PD-coated electrode. Additionally, electrode integration with a wireless sensing system provides inline monitoring via a smartphone, which can potentially be used for rapid and sensitive OA diagnosis.
摘要：
利用MnO2固定聚合物点（MnO2@D-PD）涂覆的电极设计了辅酶A（CoA-SH）响应的双电化学和荧光传感器，用于在过氧化物酶体β-氧化敲除模型中灵敏检测骨关节炎（OA）。CoA-SH响应的MnO2@D-PD涂层电极与OA软骨细胞中的CoA-SH敏感地相互作用，由于电极上的MnO2纳米片的裂解而触发电导率和荧光变化。MnO2@D-PD涂层电极可以检测未成熟关节软骨细胞原代细胞中的CoA-SH，如对照介质中电阻的显著增加(R24h=2.17MΩ)所示。该传感器还敏感地监测在乙酰辅酶A诱导剂存在下软骨细胞电阻的增加，例如植醇（Phy）和乙酸钠（SA），在介质中(R24h=2.67，3.08MΩ，分别)，与对照介质相比，证明了传感器对CoA-SH浓度增加的检测效率。此外，由于MnO2裂解，观察到荧光恢复，特别是在补充了物理和SA的培养基中。软骨细胞中OA相关的合成代谢因子（Acan）和分解代谢因子（Adamts5）的转录水平也证实了CoA-SH与MnO2@D-PD包被电极之间的相互作用。此外,电极与无线传感系统集成通过智能手机提供在线监测，可用于快速、灵敏的OA诊断。
