PDF(Pubmed)
Abstract：
UNASSIGNED: In oral and maxillofacial surgery, hard tissue augmentation is provided by materials that accelerate the healing, act as a template for reconstructing bony defects, and stimulate bone production and growth. This study investigated the effects of biocompatible carriers containing active vitamin D and platelet-rich plasma (PRP) on bone defects created in rat calvaria.
UNASSIGNED: This experimental animal study utilized fifty-two male Sprague Dawley rats divided into six groups and conducted histopathological, microtomographic, and biochemical comparisons by adding vitamin D, which has an accelerating effect on bone development. We examined the calvarial defects, sacrificing the rats in equal numbers (eight in each group and four to obtain PRP) at the first, second, fourth, and eighth weeks. The newly formed bone was assessed using histopathologic, microtomographic, and macroscopic methods as well as the biochemical analysis performed in the plasma samples. Expression of fibroblast growth factor 23 (FGF23), vitamin D receptor (VDR), and receptor activator of nuclear factor-kappa B (RANK), which determine bone formation, were investigated. The amounts of ossification, bone volume, and mineral densities were significantly higher in the fourth and eighth weeks as the biocompatible material was delivered with calcitriol.
UNASSIGNED: The histological and macroscopic examinations revealed that the defect area shrank and was colonized with new cells in the \"calcitriol + chitosan + PRP\" group. The expression of RANK, FGF23, and VDR antibodies was more intense in the \"calcitriol + chitosan + PRP\" group than in other groups of the experiment and controls.
UNASSIGNED: Active vitamin D, PRP, and chitosan formulation positively contributed to the repair of bone defects and induced remarkable clinical improvement. This new delivery approach could be promising for healing bone defects following surgical operations in hard bony tissues. HIPPOKRATIA 2023, 27 (2):48-56.
UNASSIGNED: This experimental animal study utilized fifty-two male Sprague Dawley rats divided into six groups and conducted histopathological, microtomographic, and biochemical comparisons by adding vitamin D, which has an accelerating effect on bone development. We examined the calvarial defects, sacrificing the rats in equal numbers (eight in each group and four to obtain PRP) at the first, second, fourth, and eighth weeks. The newly formed bone was assessed using histopathologic, microtomographic, and macroscopic methods as well as the biochemical analysis performed in the plasma samples. Expression of fibroblast growth factor 23 (FGF23), vitamin D receptor (VDR), and receptor activator of nuclear factor-kappa B (RANK), which determine bone formation, were investigated. The amounts of ossification, bone volume, and mineral densities were significantly higher in the fourth and eighth weeks as the biocompatible material was delivered with calcitriol.
UNASSIGNED: The histological and macroscopic examinations revealed that the defect area shrank and was colonized with new cells in the \"calcitriol + chitosan + PRP\" group. The expression of RANK, FGF23, and VDR antibodies was more intense in the \"calcitriol + chitosan + PRP\" group than in other groups of the experiment and controls.
UNASSIGNED: Active vitamin D, PRP, and chitosan formulation positively contributed to the repair of bone defects and induced remarkable clinical improvement. This new delivery approach could be promising for healing bone defects following surgical operations in hard bony tissues. HIPPOKRATIA 2023, 27 (2):48-56.
摘要：
■在口腔颌面外科，硬组织增强是由加速愈合的材料提供的，作为重建骨骼缺陷的模板，并刺激骨骼的产生和生长。这项研究调查了含有活性维生素D和富含血小板的血浆（PRP）的生物相容性载体对大鼠颅骨骨缺损的影响。
■这项实验动物研究使用了52只雄性SpragueDawley大鼠，分为六组，进行了组织病理学检查，显微层析成像,通过添加维生素D进行生化比较，对骨骼发育有加速作用。我们检查了颅骨缺损,首先以相等的数量（每组八只，四只以获得PRP）处死大鼠，第二,第四,第八周。新形成的骨使用组织病理学评估，显微层析成像,和宏观方法以及在血浆样品中进行的生化分析。成纤维细胞生长因子23（FGF23）的表达，维生素D受体(VDR),和核因子-κB受体激活剂（RANK），决定骨形成的，被调查了。骨化的数量，骨体积,在第4周和第8周，随着生物相容性材料与骨化三醇一起递送，矿物质密度显着升高。
■组织学和宏观检查显示，在“骨化三醇壳聚糖PRP”组中，缺损区域缩小并被新细胞定植。RANK的表达,在“骨化三醇壳聚糖PRP”组中，FGF23和VDR抗体的强度高于实验和对照组的其他组。
■活性维生素D,PRP,和壳聚糖制剂对骨缺损的修复有积极的贡献，并引起了显着的临床改善。这种新的递送方法有望在硬骨组织中进行外科手术后治愈骨缺损。HIPPOKRATIA2023,27(2):48-56.
■这项实验动物研究使用了52只雄性SpragueDawley大鼠，分为六组，进行了组织病理学检查，显微层析成像,通过添加维生素D进行生化比较，对骨骼发育有加速作用。我们检查了颅骨缺损,首先以相等的数量（每组八只，四只以获得PRP）处死大鼠，第二,第四,第八周。新形成的骨使用组织病理学评估，显微层析成像,和宏观方法以及在血浆样品中进行的生化分析。成纤维细胞生长因子23（FGF23）的表达，维生素D受体(VDR),和核因子-κB受体激活剂（RANK），决定骨形成的，被调查了。骨化的数量，骨体积,在第4周和第8周，随着生物相容性材料与骨化三醇一起递送，矿物质密度显着升高。
■组织学和宏观检查显示，在“骨化三醇壳聚糖PRP”组中，缺损区域缩小并被新细胞定植。RANK的表达,在“骨化三醇壳聚糖PRP”组中，FGF23和VDR抗体的强度高于实验和对照组的其他组。
■活性维生素D,PRP,和壳聚糖制剂对骨缺损的修复有积极的贡献，并引起了显着的临床改善。这种新的递送方法有望在硬骨组织中进行外科手术后治愈骨缺损。HIPPOKRATIA2023,27(2):48-56.
