PDF(Pubmed)
Abstract：
UNASSIGNED: Prenatal stress (PS) can adversely affect cognitive and psychological functions in the offspring. This study aimed to determine the effect of PS and extremely low-frequency electromagnetic field (ELF-EMF) on spatial memory, serum corticosterone, brain-derived neurotrophic factor (BDNF) concentrations, and hippocampal BDNF levels in adult male offspring.
UNASSIGNED: Female Wistar rats were randomly divided into four groups (n=6): Control, Stress, ELF-EMF (exposure to ELF-EMF), and S+EMF (simultaneous exposure to stress and the ELF-EMF) groups. Animals received interven-tions for 21 days before and 21 days during pregnancy (a total of 42 days). On the offspring\'s 90th postnatal day (PND), spatial memory was tested using Morris Water Maze, serum Corticosterone and BDNF levels were measured by the ELISA method, and hippocampal BDNF levels were measured by Western blotting.
UNASSIGNED: PS did not affect spatial memory in the adult male offspring; however, it significantly (P<0.05) increased se-rum corticosterone levels compared to the control and EMF groups. Simultaneous induction of stress with ELF-EMF disrupted the memory acquisition phase. Serum and hippocampal BDNF levels increased signifi-cantly (P<0.05) in the EMF group compared to the stress group.
UNASSIGNED: Based on our findings, PS can increase serum corticosterone levels without affecting spatial memory. Howev-er, induction of ELF-EMF with stress has a destructive effect on spatial memory with no change in the corti-costerone levels. Compared to stress, prenatal exposure to ELF-EMF increases serum and hippocampal BDNF levels. Further studies are needed to determine the underlying mechanisms of these findings.
UNASSIGNED: Female Wistar rats were randomly divided into four groups (n=6): Control, Stress, ELF-EMF (exposure to ELF-EMF), and S+EMF (simultaneous exposure to stress and the ELF-EMF) groups. Animals received interven-tions for 21 days before and 21 days during pregnancy (a total of 42 days). On the offspring\'s 90th postnatal day (PND), spatial memory was tested using Morris Water Maze, serum Corticosterone and BDNF levels were measured by the ELISA method, and hippocampal BDNF levels were measured by Western blotting.
UNASSIGNED: PS did not affect spatial memory in the adult male offspring; however, it significantly (P<0.05) increased se-rum corticosterone levels compared to the control and EMF groups. Simultaneous induction of stress with ELF-EMF disrupted the memory acquisition phase. Serum and hippocampal BDNF levels increased signifi-cantly (P<0.05) in the EMF group compared to the stress group.
UNASSIGNED: Based on our findings, PS can increase serum corticosterone levels without affecting spatial memory. Howev-er, induction of ELF-EMF with stress has a destructive effect on spatial memory with no change in the corti-costerone levels. Compared to stress, prenatal exposure to ELF-EMF increases serum and hippocampal BDNF levels. Further studies are needed to determine the underlying mechanisms of these findings.
摘要：
■产前压力（PS）会对后代的认知和心理功能产生不利影响。本研究旨在确定PS和极低频电磁场（ELF-EMF）对空间记忆的影响，血清皮质酮，脑源性神经营养因子（BDNF）浓度，和成年雄性后代海马BDNF水平。
■雌性Wistar大鼠随机分为四组（n=6）：对照组，压力,ELF-EMF（暴露于ELF-EMF），和S+EMF(同时暴露于应激和ELF-EMF)组。动物在怀孕前21天和怀孕期间21天接受干预(总共42天)。在后代出生后第90天(PND)，使用莫里斯水迷宫测试了空间记忆，ELISA法测定血清皮质酮和BDNF水平,用蛋白质印迹法测定海马BDNF水平。
■PS不会影响成年雄性后代的空间记忆；然而，与对照组和EMF组相比，它显着（P<0.05）增加了糖皮质激素水平。用ELF-EMF同时诱导应力破坏了记忆获取阶段。与应激组相比，EMF组血清和海马BDNF水平显著升高（P<0.05）。
■根据我们的发现，PS可以增加血清皮质酮水平而不影响空间记忆。但是，应激诱导ELF-EMF对空间记忆具有破坏性影响，而皮质酮水平没有变化。与压力相比，产前暴露于ELF-EMF会增加血清和海马BDNF水平。需要进一步的研究来确定这些发现的潜在机制。
■雌性Wistar大鼠随机分为四组（n=6）：对照组，压力,ELF-EMF（暴露于ELF-EMF），和S+EMF(同时暴露于应激和ELF-EMF)组。动物在怀孕前21天和怀孕期间21天接受干预(总共42天)。在后代出生后第90天(PND)，使用莫里斯水迷宫测试了空间记忆，ELISA法测定血清皮质酮和BDNF水平,用蛋白质印迹法测定海马BDNF水平。
■PS不会影响成年雄性后代的空间记忆；然而，与对照组和EMF组相比，它显着（P<0.05）增加了糖皮质激素水平。用ELF-EMF同时诱导应力破坏了记忆获取阶段。与应激组相比，EMF组血清和海马BDNF水平显著升高（P<0.05）。
■根据我们的发现，PS可以增加血清皮质酮水平而不影响空间记忆。但是，应激诱导ELF-EMF对空间记忆具有破坏性影响，而皮质酮水平没有变化。与压力相比，产前暴露于ELF-EMF会增加血清和海马BDNF水平。需要进一步的研究来确定这些发现的潜在机制。
