PDF(Pubmed)
Abstract：
UNASSIGNED: Antibiotic resistance is a global health problem related to the transmission of bacteria and genes between humans and animals. The development of new drugs with antimicrobial activity research is an urgent task of modern science.
UNASSIGNED: The article presents data of in vitro and in vivo experiments on new pharmaceutical composition based on nisin.
UNASSIGNED: The antimicrobial activity was studied on the mastitis pathogens. To identify microorganisms the Matrix-Assisted Lazer Desorption/Ionization Time-of-Flight (MALDI-TOF) (mass spectrometry) method was performed using. To determine sensitivity, the serial dilution method and the diffusion method were used. On laboratory animals, biochemical, hematological, and histological research methods were used. Female nonlinear white laboratory rats were used, which were divided into one control group and three experimental ones.
UNASSIGNED: \"Duration\" factor was statistically significant for the following indicators: hemoglobin, hematocrit, leukocytes, lymphocytes, erythrocyte sedimentation rate, and eosinophils. The \"Dose\" factor did not show significance for any indicator, which means that the effect was similar regardless of the dose chosen. When analyzing the biochemical indicators, significant differences were found in the \"Duration\" and \"Dose\" factors, in the direction of a decrease in the indicators of total protein, globulins, urea, and an increase in the concentration of alkaline phosphatase. When conducting histological studies in the first experimental group, it was established that there were no changes in the structural and functional units of the organs. In animals of the second experimental group, the presence of reversible pathological processes of a compensatory nature was noted. More profound changes in the structure of the studied organs were recorded in the third experimental group.
UNASSIGNED: An in vitro study on cell cultures showed that the pharmacological composition has high antimicrobial activity against isolates from the mammary gland secretion of cows with mastitis. An in vivo study on laboratory animals showed that the developed composition belongs to the IV class of substances \"low-hazard substances\". Histological examination made it possible to select the safest dose of the pharmacological composition of no more than 500 mg/kg.
UNASSIGNED: The article presents data of in vitro and in vivo experiments on new pharmaceutical composition based on nisin.
UNASSIGNED: The antimicrobial activity was studied on the mastitis pathogens. To identify microorganisms the Matrix-Assisted Lazer Desorption/Ionization Time-of-Flight (MALDI-TOF) (mass spectrometry) method was performed using. To determine sensitivity, the serial dilution method and the diffusion method were used. On laboratory animals, biochemical, hematological, and histological research methods were used. Female nonlinear white laboratory rats were used, which were divided into one control group and three experimental ones.
UNASSIGNED: \"Duration\" factor was statistically significant for the following indicators: hemoglobin, hematocrit, leukocytes, lymphocytes, erythrocyte sedimentation rate, and eosinophils. The \"Dose\" factor did not show significance for any indicator, which means that the effect was similar regardless of the dose chosen. When analyzing the biochemical indicators, significant differences were found in the \"Duration\" and \"Dose\" factors, in the direction of a decrease in the indicators of total protein, globulins, urea, and an increase in the concentration of alkaline phosphatase. When conducting histological studies in the first experimental group, it was established that there were no changes in the structural and functional units of the organs. In animals of the second experimental group, the presence of reversible pathological processes of a compensatory nature was noted. More profound changes in the structure of the studied organs were recorded in the third experimental group.
UNASSIGNED: An in vitro study on cell cultures showed that the pharmacological composition has high antimicrobial activity against isolates from the mammary gland secretion of cows with mastitis. An in vivo study on laboratory animals showed that the developed composition belongs to the IV class of substances \"low-hazard substances\". Histological examination made it possible to select the safest dose of the pharmacological composition of no more than 500 mg/kg.
摘要：
■抗生素耐药性是与人类和动物之间的细菌和基因传播有关的全球健康问题。开发具有抗菌活性的新药是现代科学的紧迫任务。
■本文提供了基于乳酸链球菌素的新药物组合物的体外和体内实验数据。
■对乳腺炎病原体的抗菌活性进行了研究。为了鉴定微生物,使用基质辅助Lazer解吸/电离飞行时间(MALDI-TOF)(质谱)方法进行。为了确定灵敏度，采用连续稀释法和扩散法。关于实验动物，生物化学，血液学,采用组织学研究方法。使用雌性非线性白色实验室大鼠，分为一个对照组和三个实验组。
■\“持续时间\”因素对以下指标具有统计学意义：血红蛋白，血细胞比容,白细胞,淋巴细胞，红细胞沉降率,和嗜酸性粒细胞.“剂量”因子对任何指标都没有显着性，这意味着无论选择的剂量如何，效果都是相似的。在分析生化指标时，在“持续时间”和“剂量”因素上发现了显著差异，在总蛋白质指标下降的方向上，球蛋白,尿素,碱性磷酸酶的浓度增加。在第一个实验组进行组织学研究时，确定各机构的结构和职能单位没有变化。在第二实验组的动物中，注意到存在补偿性的可逆病理过程。在第三实验组中记录了所研究器官结构的更深刻变化。
■对细胞培养物的体外研究表明，该药理组合物对来自乳腺炎奶牛乳腺分泌物的分离物具有很高的抗菌活性。对实验动物的体内研究表明，开发的组合物属于IV类物质“低危害物质”。组织学检查可以选择不超过500mg/kg的药物组合物的最安全剂量。
■本文提供了基于乳酸链球菌素的新药物组合物的体外和体内实验数据。
■对乳腺炎病原体的抗菌活性进行了研究。为了鉴定微生物,使用基质辅助Lazer解吸/电离飞行时间(MALDI-TOF)(质谱)方法进行。为了确定灵敏度，采用连续稀释法和扩散法。关于实验动物，生物化学，血液学,采用组织学研究方法。使用雌性非线性白色实验室大鼠，分为一个对照组和三个实验组。
■\“持续时间\”因素对以下指标具有统计学意义：血红蛋白，血细胞比容,白细胞,淋巴细胞，红细胞沉降率,和嗜酸性粒细胞.“剂量”因子对任何指标都没有显着性，这意味着无论选择的剂量如何，效果都是相似的。在分析生化指标时，在“持续时间”和“剂量”因素上发现了显著差异，在总蛋白质指标下降的方向上，球蛋白,尿素,碱性磷酸酶的浓度增加。在第一个实验组进行组织学研究时，确定各机构的结构和职能单位没有变化。在第二实验组的动物中，注意到存在补偿性的可逆病理过程。在第三实验组中记录了所研究器官结构的更深刻变化。
■对细胞培养物的体外研究表明，该药理组合物对来自乳腺炎奶牛乳腺分泌物的分离物具有很高的抗菌活性。对实验动物的体内研究表明，开发的组合物属于IV类物质“低危害物质”。组织学检查可以选择不超过500mg/kg的药物组合物的最安全剂量。
