PDF(Pubmed)
Abstract：
In eukaryotes, RNA splicing, an essential biological process, is crucial for precise gene expression. Inaccurate RNA splicing can cause aberrant mRNA production, disrupting protein synthesis. To regulate splicing efficiency, some splicing factors are reported to undergo Ubiquitin-like Modifier (SUMO)ylation. Our data indicate that in Saccharomyces cerevisiae, the SUMO protease, Ulp2, is involved in splicing. In the ulp2Δ mutant, some ribosomal protein (RP) transcripts exhibited a significant increase in the levels of intron-containing pre-mRNA because of improper splicing. Moreover, we confirmed Ulp2 protein binding to the intronic regions of RP genes. These findings highlight a critical Ulp2 role in RP transcript splicing.
摘要：
在真核生物中,RNA剪接,一个基本的生物过程，对精确的基因表达至关重要。不正确的RNA剪接会导致mRNA产生异常，破坏蛋白质合成.为了调节拼接效率，据报道,一些剪接因子经历泛素样修饰剂(SUMO)化。我们的数据表明，在酿酒酵母中，SUMO蛋白酶，Ulp2参与剪接。在ulp2Δ突变体中，由于剪接不当，一些核糖体蛋白（RP）转录物的含内含子的前mRNA水平显着增加。此外，我们证实了Ulp2蛋白与RP基因的内含子区结合。这些发现强调了Ulp2在RP转录物剪接中的关键作用。
