Abstract：
\'Heat-not-burn\' products (HnBP) contain lower levels of harmful substances than traditional cigarettes, but the use of these products warrants further toxicological evaluation. We have compared the cytotoxicity and genotoxicity of a heat-not burn product with conventional cigarettes, in vivo and in vitro. Male Sprague Dawley rats were exposed to mainstream smoke from conventional cigarettes or a HnBP, for 4 or 28 days, followed by isolation of bone marrow polychromatic erythrocytes (PCE) and histological examination of the testes. Chinese hamster lung fibroblast cells were exposed in vitro to total particulate matter from cigarette smoke obtained through Cambridge filters. The cytotoxicity and genotoxicity of total particulate matter were assessed by the neutral red uptake assay, chromosome aberration assay, in vitro micronucleus test, comet assay, and Ames assay. In the short-term exposure rat models, only the conventional-cigarettes group showed a significant increase in the ratio of micronuclei to total PCE. There was no significant difference in rat testis histology in the long-term exposure models. In vitro, in the neutral red uptake assay, the HnBP product showed lower cytotoxicity than conventional cigarettes. Conventional cigarettes showed greater genotoxicity in the chromosome aberration assay, high-dose Ames tests with exogenous metabolic activation, and micronucleus tests. In summary, our results suggest that HnBP have lower cytotoxicity and genotoxicity than conventional cigarettes.
摘要：
“加热不燃烧”产品（HnBP）含有比传统香烟更低的有害物质，但这些产品的使用需要进一步的毒理学评估。我们已经比较了热不燃烧产品与传统香烟的细胞毒性和遗传毒性，体内和体外。雄性SpragueDawley大鼠暴露于常规香烟或HnBP的主流烟雾中，4或28天，然后分离骨髓多染红细胞（PCE）和睾丸组织学检查。中国仓鼠肺成纤维细胞在体外暴露于通过剑桥过滤器获得的香烟烟雾中的总颗粒物。通过中性红色摄取测定法评估总颗粒物的细胞毒性和遗传毒性，染色体畸变试验,体外微核试验，彗星试验，和艾姆斯化验。在短期暴露大鼠模型中，只有常规香烟组的微核与总PCE的比率显着增加。在长期暴露模型中，大鼠睾丸组织学没有显着差异。体外，在中性红色吸收试验中，HnBP产品的细胞毒性低于传统香烟。常规香烟在染色体畸变试验中表现出更大的遗传毒性，具有外源性代谢激活的高剂量Ames测试，和微核试验.总之,我们的结果表明,HnBP比传统卷烟具有更低的细胞毒性和遗传毒性.
