Abstract：
Genetic toxicology, strategically located at the intersection of genetics and toxicology, aims to demystify the complex interplay between exogenous agents and our genetic blueprint. Telomeres, the protective termini of chromosomes, play instrumental roles in cellular longevity and genetic stability. Traditionally karyotyping and fluorescence in situ hybridisation (FISH), have been indispensable tools for chromosomal analysis following exposure to genotoxic agents. However, their scope in discerning nuanced molecular dynamics is limited. Peptide Nucleic Acids (PNAs) are synthetic entities that embody characteristics of both proteins and nucleic acids and have emerged as potential game-changers. This perspective report comprehensively examines the vast potential of PNAs in genetic toxicology, with a specific emphasis on telomere research. PNAs\' superior resolution and precision make them a favourable choice for genetic toxicological assessments. The integration of PNAs in contemporary analytical workflows heralds a promising evolution in genetic toxicology, potentially revolutionizing diagnostics, prognostics, and therapeutic avenues. In this timely review, we attempted to assess the limitations of current PNA-FISH methodology and recommend refinements.
摘要：
遗传毒理学,战略上位于遗传学和毒理学的交叉点，旨在揭开外源因子和我们的遗传蓝图之间复杂的相互作用的神秘面纱。端粒,染色体的保护性末端,在细胞寿命和遗传稳定性中起重要作用。传统的核型分析和荧光原位杂交（FISH），已成为暴露于基因毒性因子后染色体分析不可或缺的工具。然而,他们在辨别细微的分子动力学方面的范围是有限的。肽核酸（PNA）是体现蛋白质和核酸特征的合成实体，并已成为潜在的游戏规则改变者。这份透视报告全面考察了PNA在遗传毒理学方面的巨大潜力，特别强调端粒研究。PNAs卓越的分辨率和精度使其成为遗传毒理学评估的有利选择。PNA在当代分析工作流程中的整合预示着遗传毒理学的有希望的发展，潜在的革命性诊断，预测,和治疗途径。在这次及时审查中，我们试图评估目前PNA-FISH方法的局限性,并建议进行改进.
