Abstract：
The widespread use of carbamate pesticides has led to numerous environmental and health concerns, including water contamination and perturbation of endocrine homeostasis among organisms. However, there remains a paucity of research elucidating the specific effects of methomyl on gut microbial composition and physiological functions. This study aimed to investigate the intricate relationship between changes in zebrafish bacterial communities and intestinal function after 56 days of sub-chronic methomyl exposure at environmentally relevant concentrations (0, 0.05, 0.10, and 0.20 mg/L). Our findings reveal significant methomyl-induced morphological changes in zebrafish intestines, characterized by villi shortening and breakage. Notably, methomyl exposure down-regulated nutrient and energy metabolism, and drug metabolism at 0.05-0.10 mg/L, while up-regulating cortisol, inflammation-related genes, and apoptotic markers at 0.20 mg/L. These manifestations indicate physiological stress imposition and disruption of gut microbiota equilibrium, impacting metabolic processes and instigating low-grade inflammatory responses and apoptotic cascades. Importantly, changes in intestinal function significantly correlated with shifts in specific bacterial taxa abundance, including Shewanella, Rubrobacter, Acinetobacter, Bacillus, Luteolibacter, Nocardia, Defluviimonas, and Bacteroides genus. In summary, our study underscores the potential adverse effects of environmental methomyl exposure on aquatic organisms, emphasizing the necessity for further research to mitigate its repercussions on environmental health and ecosystem stability.
摘要：
氨基甲酸酯类农药的广泛使用引起了众多的环境和健康问题,包括水污染和生物之间内分泌稳态的干扰。然而,关于灭多威对肠道微生物组成和生理功能的具体影响的研究仍然很少。本研究旨在调查环境相关浓度（0、0.05、0.10和0.20mg/L）的亚慢性灭多威暴露56天后斑马鱼细菌群落变化与肠道功能之间的复杂关系。我们的发现揭示了在斑马鱼肠道中显著的灭多威诱导的形态学变化,以绒毛缩短和断裂为特征。值得注意的是,灭多威暴露下调营养和能量代谢，和药物代谢在0.05-0.10mg/L，在上调皮质醇的同时，炎症相关基因，和凋亡标志物在0.20mg/L。这些表现表明生理应激强加和肠道微生物群平衡的破坏，影响代谢过程并激发低度炎症反应和凋亡级联反应。重要的是,肠道功能的变化与特定细菌分类群丰度的变化显着相关，包括Shewanella,鲁布杆菌,不动杆菌,芽孢杆菌,Luteolibacter,诺卡氏菌,去氟单胞菌,和拟杆菌属。总之,我们的研究强调了环境接触灭多威对水生生物的潜在不利影响，强调需要进一步研究以减轻其对环境健康和生态系统稳定性的影响。
