Abstract：
We investigated the influence of archaeal lipids (C25,25) isolated from thermophilic archaeon Aeropyrum pernix K1 on physicochemical properties of liposomes comprised of egg sphingomyelin (SM) and cholesterol (CH) using fluorescence emission anisotropy, calcein release studies, dynamic light scattering, transmission electron microscopy and phase analysis light scattering. The 2 mol% addition of archaeal lipids enabled formation of small unilamellar vesicles by sonication while also having significant effect on reducing mean size, polydispersity index and zeta potential of C25,25/SM/CH vesicles. Increasing the ratio of C25,25 lipids in mixture of C25,25/SM/CH decreased lipid ordering parameter in dose dependent manner at different temperatures. We also demonstrated that adding 15 mol% C25,25 to SM/CH mixture will cause it to notably interact with fetal bovine serum which could make them a viable alternative adjuvant to synthetic ether-linked lipids in development of advanced liposomal vaccine delivery systems. The prospect of combining the proven strengths of SM/CH mixtures with the unique properties of C25,25 opens exciting possibilities for advancing drug delivery technologies, promising to yield formulations that are both highly effective and adaptable to a range of therapeutic applications.
摘要：
我们使用荧光发射各向异性研究了从嗜热古细菌AeropyrumpernixK1分离的古细菌脂质（C25,25）对由卵鞘磷脂（SM）和胆固醇（CH）组成的脂质体的理化性质的影响，钙黄绿素释放研究，动态光散射,透射电子显微镜和相位分析光散射。添加2mol％的古细菌脂质可以通过超声处理形成小的单层囊泡，同时对减小平均大小也有显着影响。C25,25/SM/CH囊泡的多分散指数和ζ电位。在不同温度下，增加C25,25/SM/CH混合物中C25,25脂质的比例以剂量依赖性方式降低了脂质排序参数。我们还证明，向SM/CH混合物中添加15mol％C25,25将使其与胎牛血清显着相互作用，这可能使它们成为开发高级脂质体疫苗递送系统中合成醚连接脂质的可行替代佐剂。将SM/CH混合物的成熟优势与C25,25的独特特性相结合的前景为推进药物输送技术开辟了令人兴奋的可能性，有望产生既高效又适用于一系列治疗应用的制剂。
