Abstract：
Three-dimensional cell spheroids show promise for the reconstruction of native tissues. Herein, we report a sophisticated, uniform, and highly reproducible spheroid culture system for tissue reconstruction. A mesh-integrated culture system was designed to precisely control the uniformity and reproducibility of spheroid formation. Furthermore, we synthesized hexanoyl glycol chitosan, a material with ultralow cell adhesion properties, to further improve spheroid formation efficiency and biological function. Our results demonstrate improved biological function in various types of cells and ability to generate spheroids with complex structures composed of multiple cell types. In conclusion, our spheroid culture system offers a highly effective and widely applicable approach to generating customized spheroids with desired structural and biological features for a variety of biomedical applications.
摘要：
三维细胞球体显示出重建天然组织的希望。在这里，我们报告了一个复杂的，制服,和高度可重复的球体培养系统，用于组织重建。设计了网状集成培养系统，以精确控制球状体形成的均匀性和可重复性。此外，我们合成了己二醇壳聚糖，一种具有超低细胞粘附特性的材料，进一步提高球状体形成效率和生物功能。我们的结果表明，在各种类型的细胞中具有改善的生物学功能，并且能够产生具有由多种细胞类型组成的复杂结构的球体。总之,我们的球体培养系统提供了一个非常有效和广泛适用的方法来产生定制的球体具有所需的结构和生物学特征的各种生物医学应用。
