PDF(Pubmed)
Abstract：
The research focused on analyzing the UGT gene family in Cannabis sativa, which plays a crucial role in the plant\'s metabolism and glycosylation of secondary metabolites. The study identified 125 UGTs using conserved plant secondary product glycosyltransferase (PSPG) motif amino acid sequences. These UGT genes were categorized into 17 groups (A-Q) through phylogenetic analysis, showing their distribution across 10 chromosomes in C. sativa. The expansion of the CsUGT gene family was attributed to tandem and duplication events, as suggested by gene duplication analysis. Furthermore, the study found various cis-acting regulatory elements related to phytohormones and stress responses in CsUGT promoter regions. Subcellular localization analysis revealed that CsUGT is present in the cytoplasm, chloroplast, and nucleus. The study revealed that CsUGT plays a significant role in various biological processes, cellular components, and molecular functions as highlighted by Gene Ontology analysis. Additionally, the Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis showed that some CsUGTs are associated with the biosynthesis of secondary metabolites. This research provides valuable insights into the genomic organization, evolutionary history, and potential regulatory mechanisms of UGT genes in C. sativa. It lays the foundation for further exploration of their specific biological roles and potential applications in the plant\'s metabolism and stress responses. These findings contribute to a better understanding of the UGT gene family and its relevance to the metabolic pathways in C. sativa.
UNASSIGNED: The online version contains supplementary material available at 10.1007/s13205-024-04025-3.
UNASSIGNED: The online version contains supplementary material available at 10.1007/s13205-024-04025-3.
摘要：
本研究的重点是分析大麻UGT基因家族，在植物的代谢和次生代谢产物的糖基化中起着至关重要的作用。该研究使用保守的植物次级产物糖基转移酶（PSPG）基序氨基酸序列鉴定了125个UGT。这些UGT基因通过系统发育分析分为17组（A-Q），显示它们在水稻10条染色体上的分布。CsUGT基因家族的扩展归因于串联和重复事件，正如基因复制分析所表明的。此外，研究发现CsUGT启动子区存在多种与植物激素和应激反应相关的顺式调控元件。亚细胞定位分析显示CsUGT存在于细胞质中，叶绿体，和核。研究表明，CsUGT在各种生物过程中发挥着重要作用，细胞成分，和基因本体论分析强调的分子功能。此外,京都基因和基因组百科全书（KEGG）途径分析表明，一些CsUGTs与次级代谢产物的生物合成有关。这项研究为基因组组织提供了有价值的见解，进化史,以及紫花苜蓿UGT基因的潜在调控机制。为进一步探索它们在植物代谢和逆境响应中的具体生物学作用和潜在应用奠定了基础。这些发现有助于更好地理解UGT基因家族及其与水稻代谢途径的相关性。
■在线版本包含补充材料，可在10.1007/s13205-024-04025-3获得。
■在线版本包含补充材料，可在10.1007/s13205-024-04025-3获得。
