PDF(Pubmed)
Abstract：
Introduction: Cissus quadrangularis is a vining plant widely used as a traditional herbal remedy for various ailments. In this study, the therapeutic effects of C. quadrangularis extract (CQR-300) on type 2 diabetes mellitus (T2DM) were investigated in a leptin receptor-mutated db/db mouse model. Methods: CQR-300 was orally administered to db/db mice (n = 6/group) at different doses (50, 100, and 200 mg/kg) for 8 weeks. Blood glucose levels and oral glucose tolerance were assessed using the AccuCheck glucometer. Enzyme-linked immunosorbent assay was performed to evaluate insulin and hemoglobin A1c (HbA1c) levels in the blood of db/db mice. Liver and pancreatic tissues from db/db mice were examined by hematoxylin and eosin (H&E) and immunohistochemical staining. The protein levels of gluconeogenesis-, lipogenesis-, and oxidative stress-related factors were evaluated using western blotting. Results and discussion: CQR-300 treatment effectively reduced body weight, blood glucose, and insulin levels. HbA1c levels were increased by leptin receptor mutation. Additionally, in the oral glucose tolerance tests, the CQR-300 treated group had a faster blood glucose recovery rate than the db/db group. H&E and Oil red-O staining of the liver showed decreased lipid accumulation in the CQR-300 treated group than the db/db group. Western blot analysis confirmed that CQR-300 effectively inhibited gluconeogenesis, lipogenesis, and oxidative stress-related factors. Our findings suggest that CQR-300 has the potential to be used as a T2DM supplement.
摘要：
简介：Cissusquadrangularis是一种酿酒植物，广泛用作各种疾病的传统草药。在这项研究中,在瘦素受体突变的db/db小鼠模型中研究了四角梭菌提取物(CQR-300)对2型糖尿病(T2DM)的治疗作用.方法:对db/db小鼠(n=6/组)以不同剂量(50、100和200mg/kg)口服CQR-300,持续8周。使用AccuCheck血糖仪评估血糖水平和口服葡萄糖耐量。进行酶联免疫吸附测定以评估db/db小鼠血液中的胰岛素和血红蛋白A1c（HbA1c）水平。通过苏木精和曙红(H&E)和免疫组织化学染色检查db/db小鼠的肝和胰腺组织。糖异生的蛋白质水平-，脂肪基因-，和氧化应激相关因素使用蛋白质印迹进行评估。结果与讨论：CQR-300治疗可有效降低体重，血糖，和胰岛素水平。瘦素受体突变导致HbA1c水平升高。此外,在口服葡萄糖耐量试验中，CQR-300治疗组比db/db组有更快的血糖恢复率.肝脏的H&E和油红-O染色显示,与db/db组相比,CQR-300处理组中的脂质积累减少。Westernblot分析证实，CQR-300有效抑制糖异生，脂肪生成,和氧化应激相关因素。我们的研究结果表明，CQR-300有可能作为T2DM的补充。
