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Abstract：
The efficiency of human immunodeficiency virus-1 (HIV-1) inhibition by sulfated polysaccharides isolated from the various families of red algae of the Far East Pacific coast were studied. The anti-HIV-1 activity of kappa and lambda-carrageenans from Chondrus armatus, original highly sulfated X-carrageenan with low content of 3,6-anhydrogalactose from Tichocarpus crinitus and i/κ-carrageenan with hybrid structure isolated from Ahnfeltiopsis flabelliformis was found. The antiviral action of these polysaccharides and its low-weight oligosaccharide was compared with commercial κ-carrageenan. Here we used the HIV-1-based lentiviral particles and evaluated that these carrageenans in non-toxic concentrations significantly suppress the transduction potential of lentiviral particles pseudotyped with different envelope proteins, targeting cells of neuronal or T-cell origin. The antiviral action of these carrageenans was confirmed using the chimeric replication competent Mo-MuLV (Moloney murine leukemia retrovirus) encoding marker eGFP protein. We found that X-carrageenans from T. crinitus and its low weight derivative and λ-carrageenan from C. armatus effectively suppress the infection caused by retrovirus. The obtained data suggest that the differences in the suppressive effect of carrageenans on the transduction efficiency of HIV-1 based lentiviral particles may be related to the structural features of the studied polysaccharides.
摘要：
研究了从远东太平洋沿岸各个红藻家族分离的硫酸化多糖对人类免疫缺陷病毒1（HIV-1）的抑制作用。来自Chondrusarmatus的κ和λ-角叉菜胶的抗HIV-1活性，发现了原始的高度硫酸化的X-角叉菜胶，其中3,6-脱水半乳糖含量低，来自Tichocarpuscrinitus和i/κ-角叉菜胶，具有杂化结构，从松弛的白蚁中分离。将这些多糖及其低重量寡糖的抗病毒作用与市售κ-角叉菜胶进行了比较。在这里，我们使用了基于HIV-1的慢病毒颗粒，并评估了这些角叉菜胶在无毒浓度下显着抑制具有不同包膜蛋白假型化的慢病毒颗粒的转导潜力。靶向神经元或T细胞来源的细胞。使用编码标记eGFP蛋白的嵌合复制能力Mo-MuLV(莫洛尼鼠白血病逆转录病毒)证实这些角叉菜胶的抗病毒作用。我们发现来自T.crinitus的X-角叉菜胶及其低重量衍生物和来自C.armatus的λ-角叉菜胶有效地抑制由逆转录病毒引起的感染。获得的数据表明，角叉菜胶对基于HIV-1的慢病毒颗粒的转导效率的抑制作用的差异可能与所研究的多糖的结构特征有关。
