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Abstract：
Neospora spp. and Toxoplasma gondii are two closely related protozoan parasites that are widely distributed throughout the world. Horses can act as intermediate hosts for both parasites and can acquire disease. Blood samples were taken from 487 clinically healthy horses from 17 different mechanized stables in Tehran, the capital of Iran, during September and November of 2022. IFAT and ELISA were employed to detect antibodies directed against Neospora spp. and T. gondii. The anti-N. caninum antibodies were detected in 52 of the horses (10.67%) based on IFAT and in 86 of the 487 horses (17.65%) based on the ELISA test. Also, antibodies against T. gondii were detected in 41 horses (8.42%) based on IFAT and in 63 of 487 horses (12.94%) based on the ELISA test. Also, in 6 of the horses (1.23%) based on IFAT and in 13 of the 487 horses (2.67%) based on the ELISA test, double positivity suggested co-infection with both parasites. Gender, age groups, and the presence of dogs for neosporosis, and age groups and the presence of cats for toxoplasmosis, could be considered factors having an influence on the seroprevalences (P < 0.05). The results proved the importance of the urgent implementation of stringent regulatory measures to prevent and control the spread of these parasites.
摘要：
新孢子菌属。弓形虫和弓形虫是两种密切相关的原生动物寄生虫，广泛分布于世界各地。马可以充当两种寄生虫的中间宿主，并可以获得疾病。血液样本取自德黑兰17个不同机械化马厩的487匹临床健康马匹，伊朗首都,2022年9月和11月。IFAT和ELISA用于检测针对新孢子菌属的抗体。还有T.Gondii.反N.根据IFAT，在52匹马（10.67％）中检测到犬抗体，根据ELISA测试，在487匹马中的86匹马（17.65％）中检测到犬抗体。此外，基于IFAT的41匹马（8.42％）和基于ELISA测试的487匹马中的63匹马（12.94％）检测到针对弓形虫的抗体。此外，基于IFAT的6匹马（1.23％）和基于ELISA测试的487匹马中的13匹马（2.67％），双阳性提示与两种寄生虫共感染。性别,年龄组,以及新孢子虫病的狗的存在，年龄组和弓形虫病的猫的存在，可以认为是对血清效价有影响的因素（P<0.05）。结果证明了紧急实施严格的监管措施以预防和控制这些寄生虫传播的重要性。
