Abstract：
Glycosylation is the most prominent modification important for vaccines and its specific pattern depends on several factors that need to be considered when developing a new biopharmaceutical. Tailor-made glycosylation can be exploited to develop more effective and safer vaccines; for this reason, a deep understanding of both glycoengineering strategies and glycans structures and functions is required. In this review we discuss the recent advances concerning glycoprotein expression systems and the explanation of glycans immunomodulation mechanisms. Furthermore, we highlight how glycans tune the immunological properties among different vaccines platforms (whole virus, recombinant protein, nucleic acid), also comparing commercially available formulations and describing the state-of-the-art analytical technologies for glycosylation analysis. The whole review stresses the aspect of glycoprotein glycans as a potential tool to overcome nowadays medical needs in vaccine field.
摘要：
糖基化是对疫苗最重要的修饰，其具体模式取决于开发新生物药物时需要考虑的几个因素。可以利用量身定制的糖基化来开发更有效和更安全的疫苗；因此，需要对糖工程策略和聚糖结构和功能有深入的了解。在这篇综述中，我们讨论了有关糖蛋白表达系统的最新进展以及聚糖免疫调节机制的解释。此外，我们强调了聚糖如何在不同的疫苗平台(整个病毒，重组蛋白，核酸)，还比较了市售制剂并描述了用于糖基化分析的最新分析技术。整个综述强调了糖蛋白聚糖作为克服疫苗领域当今医学需求的潜在工具的方面。
