Abstract：
Worldwide, female breast cancer (BC) has surpassed lung cancer as the most commonly diagnosed cancer. Early diagnosis of cancer recurrence can provide substantial benefits for BC patients who are at high risk of relapse. We aimed to investigate the role of ALU 247, ALU 115, cfDNA integrity index, CA15-3 and CEA as potential diagnostic markers in BC patients and as markers for early prediction of recurrence. Fifty BC patients (10 patients showed recurrence), 26 BBD patients and 22 healthy controls were included. Real-time q-PCR was used to measure the concentration of ALU 247 and ALU 115 in plasma then cfDNA integrity index was calculated. \"ECLIA\" was used to measure the concentration of CA15-3 and CEA in serum. Our results showed significant higher levels of ALU 247, ALU 115, CA15-3 and CEA in BC patients in comparison to healthy controls (P=0.02, 0.008, <0.001 and < 0.001 respectively). Also, cfDNA integrity index was higher in BC patients in comparison to healthy controls but statistically insignificance (p = 0.46). In recurrent BC patients; ALU 247, ALU 115, cfDNA integrity index, CA15-3 and CEA levels were higher compared to non-recurrent BC patients but with no statistic significant (p = 0.46, 0.59, 0.09, 0.85 and 0.84 respectively). This may result from the short period of follow up (1-2 years) and the relatively small sample size due to exclusion of patients with chronic diseases or inflammation as well as those who received therapy or post-surgery. By using the ROC curve, the sensitivity of ALU 247, ALU 115, CA15-3 and CEA for discriminating BC patients from BBD patients and healthy controls was 79 %, 79.2 %, 76.0 % and 88.0 % respectively. This study suggested that ALU 247, ALU 115, CA15-3 and CEA could be promising non-invasive markers of BC for diagnosis and early prediction of recurrence after validation in large-scale future studies.
摘要：
全球,女性乳腺癌（BC）已超过肺癌，成为最常见的诊断癌症。癌症复发的早期诊断可以为处于高复发风险的BC患者提供实质性益处。我们的目的是调查ALU247，ALU115，cfDNA完整性指数的作用，CA15-3和CEA作为BC患者的潜在诊断标志物和作为早期预测复发的标志物。50例BC患者（10例复发），纳入26例BBD患者和22例健康对照。使用实时q-PCR测量血浆中ALU247和ALU115的浓度,然后计算cfDNA完整性指数。“ECLIA”用于测量血清中CA15-3和CEA的浓度。我们的结果显示，与健康对照组相比，BC患者的ALU247，ALU115，CA15-3和CEA水平显着升高（分别为P=0.02，0.008，<0.001和<0.001）。此外，与健康对照组相比，BC患者的cfDNA完整性指数较高，但统计学意义不大（p=0.46）。在复发性BC患者中；ALU247，ALU115，cfDNA完整性指数，与非复发性BC患者相比，CA15-3和CEA水平更高，但没有统计学意义（分别为p=0.46、0.59、0.09、0.85和0.84）。这可能是由于短期随访（1-2年）和由于排除了患有慢性疾病或炎症的患者以及接受治疗或手术后的患者而导致的相对较小的样本量。通过使用ROC曲线，ALU247，ALU115，CA15-3和CEA区分BBD患者和健康对照组的敏感性为79％，79.2%,分别为76.0%和88.0%。这项研究表明，ALU247，ALU115，CA15-3和CEA可能是有希望的非侵入性BC标志物，用于在大规模未来研究中验证后诊断和早期预测复发。
