Abstract：
RNA-RNA interactions (RRIs) can dictate RNA molecules to form intricate higher-order structures and bind their RNA substrates in diverse biological processes. To elucidate the function, binding specificity, and regulatory mechanisms of various RNA molecules, especially the vast repertoire of non-coding RNAs, advanced technologies and methods that globally map RRIs are extremely valuable. In the past decades, many state-of-the-art technologies have been developed for this purpose. This review focuses on those high-throughput technologies for the global mapping of RRIs. We summarize the key concepts and the pros and cons of different technologies. In addition, we highlight the novel biological insights uncovered by these RRI mapping methods and discuss the future challenges for appreciating the crucial roles of RRIs in gene regulation across bacteria, viruses, archaea, and mammals.
摘要：
RNA-RNA相互作用（RRIs）可以指示RNA分子形成复杂的高阶结构，并在不同的生物过程中结合它们的RNA底物。为了阐明功能，结合特异性，以及各种RNA分子的调控机制，尤其是大量的非编码RNA，全球映射RRI的先进技术和方法非常有价值。在过去的几十年里，为此开发了许多最先进的技术。这篇综述的重点是那些用于RRI全球映射的高通量技术。我们总结了关键概念和不同技术的优缺点。此外,我们强调了这些RRI作图方法发现的新生物学见解，并讨论了认识RRI在细菌基因调控中的关键作用的未来挑战，病毒,古细菌,和哺乳动物。
