Abstract：
Heavy metal Cu2+ emitted in industry and residues of glyphosate pesticides are pervasive in ecosystems, accumulated in water bodies and organisms\' overtime, constituting hazard to human and ecological balance. The development of rapid, highly selective, reversibility and sensitive biosensor in vivo detection for Cu2+ and glyphosate was imminent. A novel dual-recognition fluorescence biosensor MPH was successfully synthesized based on triphenylamine, which demonstrated remarkable ratiometric fluorescence quenching toward Cu2+, while MPH-Cu2+ (1:1) ensemble exhibited ratiometric fluorescence restoration for glyphosate, both with observable color changes in daylight and UV lamp. The biosensor exhibited rapid, outstanding selectivity, anti-interference, and multiple cycles reversibility through \"turn-off-on\" fluorescence towards Cu2+ and glyphosate, respectively. Surprisingly, the clearly binding mechanisms of MPH to Cu2+ and MPH-Cu2+ ensemble to glyphosate were determined, respectively, based on the Job\'s plot, FT-IR, ESI-HRMS, 1H NMR titration and theoretical calculations of dynamics and thermodynamics. In addition, biosensor MPH demonstrated successful detection of Cu2+ and glyphosate across diverse environmental samples including tap water, extraction solutions of traditional Chinese medicine honeysuckle and soil samples. In the meantime, fluorescence imaging of Cu2+ and glyphosate at both micro and macro scales in various living organisms, such as rice roots, MCF-7 cells, zebrafish, and mice, were successfully achieved. Overall, this work was expected to become a promising and versatile fluorescence biosensor for rapid and reversible detection of Cu2+ and glyphosate both in vitro and vivo.
摘要：
工业中排放的重金属Cu2+和草甘膦农药残留在生态系统中普遍存在，积累在水体和有机体中，对人类和生态平衡构成危害。发展迅速,高度选择性,生物传感器体内检测Cu2+和草甘膦的可逆性和灵敏性迫在眉睫。成功合成了基于三苯胺的新型双识别荧光生物传感器MPH,这表明对Cu2+显著的比率荧光猝灭，而MPH-Cu2+(1:1)集合对草甘膦表现出比率荧光恢复，在日光和紫外线灯中都有明显的颜色变化。生物传感器表现出快速，出色的选择性，抗干扰,通过“关闭-开启”荧光对Cu2+和草甘膦的多次循环可逆性，分别。令人惊讶的是,确定了MPH与Cu2+和MPH-Cu2+与草甘膦的明显结合机制，分别,根据作业的情节，FT-IR,ESI-HRMS,1HNMR滴定及动力学和热力学理论计算。此外,生物传感器MPH证明了Cu2+和草甘膦的成功检测在不同的环境样品，包括自来水，中药金银花和土壤样品的提取溶液。同时,Cu2+和草甘膦在各种生物体的微观和宏观尺度的荧光成像，如水稻根，MCF-7细胞,斑马鱼,和老鼠，已成功实现。总的来说,这项工作有望成为一个有前途的和通用的荧光生物传感器，用于快速和可逆检测Cu2+和草甘膦在体外和体内。
