Abstract：
Chemotherapy-induced intestinal mucositis based on 5-fluorouracil (5-FU) slows down the progress of cancer treatment and causes significant suffering to patients. Pediococcus pentosaceus (P. pentosaceus), as a type of LAB, has a range of probiotic properties, including antioxidant, immune benefits, and cholesterol-lowering effects, which are attracting increasing attention. However, studies on the protective effect of P. pentosaceus against chemotherapeutic-induced intestinal mucositis caused by 5-FU remain unclear. Therefore, this study aimed to investigate the potential relieving effects of P. pentosaceus PP34 on 5-FU-induced intestinal mucositis and its mechanism. In the present study, a P. pentosaceus PP34 solution (2 × 109 CFU/mL) was administered daily by gavage followed by intraperitoneal injection of 5-FU to model intestinal mucositis. The body weight, serum biochemical indices, jejunal pathological organization, and expression levels of inflammatory cytokines in the jejunum were examined. The results indicated that the mice induced with 5-FU developed typical intestinal mucositis symptoms and histopathological changes with intense inflammatory and oxidative responses. Moreover, the gut microbiota was disturbed, while PP34 effectively decreased the oxidative reactions and the expression levels of inflammatory mediators and regulated the gut microbiota in 5-FU-exposed mice. Taken together, the study indicated that P. pentosaceus PP34 ameliorates 5-Fluorouracil-induced intestinal mucositis via inhibiting oxidative stress and restoring the gut microbiota.
摘要：
基于5-氟尿嘧啶(5-FU)的化疗引起的肠粘膜炎减慢了癌症治疗的进展,并给患者带来显著痛苦。戊糖片球菌(P.pentosacus),作为一种实验室，有一系列的益生菌特性，包括抗氧化剂，免疫益处，和降低胆固醇的作用，正引起越来越多的关注。然而,关于戊糖对5-FU引起的化疗诱导的肠黏膜炎的保护作用的研究仍不清楚。因此,本研究旨在探讨戊糖PP34对5-FU诱导的肠黏膜炎的潜在缓解作用及其机制。在本研究中,posososacusPP34溶液（2×109CFU/mL）每天通过管饲法给药，然后腹膜内注射5-FU来模拟肠粘膜炎。体重,血清生化指标,空肠病理组织，检查空肠中炎性细胞因子的表达水平。结果表明，5-FU诱导的小鼠出现典型的肠粘膜炎症状和组织病理学变化，并伴有强烈的炎症和氧化反应。此外，肠道微生物群受到干扰，而PP34有效降低了5-FU暴露小鼠的氧化反应和炎症介质的表达水平，并调节了肠道菌群。一起来看,这项研究表明，posososacusPP34通过抑制氧化应激和恢复肠道微生物区来改善5-氟尿嘧啶诱导的肠黏膜炎。
