Abstract：
ER:YAG laser and experimental resin-based dental adhesive loaded with functionalized carbonated apatite filler were used in this study to evaluate the dentin interaction in terms of penetration and occlusion of the dentinal tubules aiding in the control of dentin hypersensitivity (DH). Spheroidal Carbonated apatite nanoparticles (N-CAP), with an average size of 20±5 nm diameter, were synthesized, characterized, and incorporated in a universal adhesive \"All Bond Universal, Bisco, USA\", in (2% weight) concentration. Er:YAG laser \"Lightwalker, FOTONA, EU\" was adjusted to an energy output of 40mJ/ pulse and pulse repetition of 10 Hz for 10 seconds. Dentin specimens were prepared from the buccal surface of 75 extracted sound human molars. The specimens were randomly divided into five groups (n=15) according to the surface treatment: Group (L): Laser only; Group (LB): Laser in combination with adhesive; Group (LBN): Laser in combination with adhesive loaded with N-CAP; Group (B): adhesive only; and Group (BN): adhesive loaded with N-CAP. Depth of penetration and occlusion of the dentinal tubules were assessed using Environmental Scanning Electron Microscope Examination (ESEM). One-way ANOVA was used to compare groups, followed by a pairwise test for multiple comparisons (α=0.05). Groups (LB), and (LBN) showed the highest mean of dentinal tubules\' penetration, with a non-significant difference between them. In contrast, the specimens treated with laser only (L) showed the most minor penetration. The employment of ER-YAG laser irradiation with the adhesive loaded with N-CAP was evaluated to be effective in penetrating and occluding the opened dentinal tubules.
摘要：
ER:在本研究中使用YAG激光和负载有官能化碳酸化磷灰石填料的基于树脂的实验性牙科粘合剂,以评估牙本质相互作用,在牙本质小管的渗透和闭塞方面帮助控制牙本质过敏(DH)。球团碳酸化磷灰石纳米颗粒(N-CAP),平均直径为20±5nm，是合成的，characterized,并结合在通用粘合剂中，Bisco,美国\",在（2％重量）浓度。Er:YAG激光\"Lightwalker,FOTONA,将EU“调整为40mJ/脉冲的能量输出和10Hz的脉冲重复10秒。牙本质标本是从75个提取的声音人类磨牙的颊表面制备的。根据表面处理将样品随机分为五组(n=15):组(L):仅激光;组(LB):激光与粘合剂组合;组(LBN):激光与负载有N-CAP的粘合剂组合;组(B):仅粘合剂;和组(BN):负载有N-CAP的粘合剂。使用环境扫描电子显微镜检查（ESEM）评估牙本质小管的穿透和闭塞深度。单因素方差分析用于比较各组，然后进行多重比较的成对检验(α=0.05)。团体(LB),（LBN）显示出最高的牙本质小管渗透平均值，它们之间的差异不显著。相比之下,用激光处理的标本（L）显示出最轻微的渗透。评估了使用ER-YAG激光辐照与负载有N-CAP的粘合剂的情况下，可以有效地穿透和闭塞打开的牙本质小管。
