Abstract:
Chironomusnuditarsis Keyl, 1961 is recorded from Sevan Lake for the first time. This species is widespread in Europe, the Caucasus, and Siberia. For species identification, we used a comprehensive approach that included morphological, cytogenetic and molecular genetic analyses. Morphological analysis showed a high similarity with the description. Nine chromosome banding sequences ndtA1, ndtA2, ndtB2, ndtC1, ndtD1, ndtE1, ndtF1, ndtG1, and ndtG2 were found. The banding sequences ndtA1, ndtA2, ndtG1, and ndtG2 are species-specific for C.nuditarsis and allow us to accurately distinguish it from the sibling species Ch.curabilis Belyanina, Sigareva et Loginova, 1990. Molecular-genetic analysis of the COI gene sequences has shown low genetic distances of 0.38-0.95% in the sibling species Ch.nuditarsis and Ch.curabilis complex and the insufficiency of using a single COI as a molecular marker for their separation.
摘要:
扭动眼,1961年是第一次从塞万湖录制。这个物种在欧洲很普遍,高加索,和西伯利亚。为了物种鉴定,我们使用了一种全面的方法,包括形态学,细胞遗传学和分子遗传学分析。形态学分析显示与描述有很高的相似性。发现了9个染色体条带序列ndtA1,ndtA2,ndtB2,ndtC1,ndtD1,ndtE1,ndtF1,ndtG1和ndtG2。条带序列ndtA1,ndtA2,ndtG1和ndtG2是对女性的物种特异性的,使我们能够将其与同胞物种Ch准确区分开。curabilisBelyanina,SigarevaetLoginova,1990.COI基因序列的分子遗传分析表明,同胞物种Ch的低遗传距离为0.38-0.95%。女声和Ch。curabilis复杂,无法使用单个COI作为分子标记进行分离。