PDF(Pubmed)
Abstract：
The lack of diagnostic markers limits the window of effectiveness for rheumatoid arthritis (RA) therapies. Here, we isolated exosomes of serum samples from four distinct groups RA patients, according to disease activity and with/without medication. Then, total RNA of exosomes was extracted for whole-transcriptome sequencing. Focusing on lncRNA sequencing, gene ontology (GO) and kyoto encyclopedia of genes and genomes (KEGG) pathway enrichment analyses were performed. We found that the number of upregulated lncRNAs were significantly higher than that of downregulated lncRNAs in each four RA groups. And most importantly, we identified two specific lncRNAs from differentially expressed lncRNAs, TCONS_I2_00013502 (up-regulated) and ENST00000363624 (down-regulated) in RA. Receiver Operating Characteristic (ROC) curve analysis showed that the two lncRNAs were promising biomarkers for RA diagnosis. These findings highlight lncRNAs of the serum exosome are important biomarkers and provide application potential for diagnosis of RA.
摘要：
诊断标记物的缺乏限制了类风湿性关节炎(RA)治疗的有效性窗口。这里,我们从四组不同的RA患者中分离出血清外泌体，根据疾病活动和有/没有药物治疗。然后，提取外泌体的总RNA用于全转录组测序.专注于lncRNA测序，进行了基因本体论（GO）和京都基因和基因组百科全书（KEGG）途径富集分析。我们发现，在每四个RA组中，上调的lncRNAs的数量明显高于下调的lncRNAs的数量。最重要的是,我们从差异表达的lncRNAs中鉴定出两个特定的lncRNAs，RA中的TCONS_I2_00013502(上调)和ENST00000363624(下调)。受试者工作特征（ROC）曲线分析显示，两种lncRNA是RA诊断的有希望的生物标志物。这些发现强调了血清外泌体的lncRNAs是重要的生物标志物，并为RA的诊断提供了应用潜力。
