PDF(Pubmed)
Abstract：
The absence of improvement in survival rates across various cancers, including laryngeal cancer, has led to an increasing interest in understanding the immune response to cancer. In head and neck cancers, immune modulatory mechanisms such as immune microenvironment and immune infiltration are important in cancer pathogenesis. This study aims to explore the distribution of tumor-infiltrating lymphocyte (TIL) subgroups in the immune microenvironment and evaluate their impact on tumor histopathological characteristics and prognosis. The study included 50 patients who underwent laryngectomy for laryngeal squamous cell carcinoma, in Istanbul University - Cerrahpaşa, Faculty of Medicine Department of Otorhinolaryngology, between January 2016 and January 2018. Pathology specimens were evaluated using immunohistochemistry to assess the expressions of the CD3, CD20, CD8, CD4, CD25, and FoxP3 markers, identifying subgroups of TILs. The investigation aimed to uncover how these subgroups influence tumor histopathological features and survival outcomes. The high infiltration of CD3, CD20, and CD4 had a positive impact on disease-specific survival, disease-free survival, and recurrence-free survival. In addition, overall survival was positively affected by high CD3 and CD4 infiltrations. However, no significant relationship was observed between the expressions of CD8, FoxP3, and CD25 and any of the survival parameters. The infiltration of CD3, CD20, and CD4 positive cells indicative of a robust antitumoral immune response-emerged as favorable prognostic factors in laryngeal cancer. These findings suggest that enhancing the infiltration of CD3, CD20, and CD4 lymphocytes could be a therapeutic strategy worth exploring in clinical trials.
摘要：
各种癌症的存活率没有提高，包括喉癌,引起了人们对了解癌症免疫反应的兴趣。在头颈部癌症中，免疫微环境和免疫浸润等免疫调节机制在肿瘤发病机制中具有重要意义。本研究旨在探讨肿瘤浸润淋巴细胞(TIL)亚群在免疫微环境中的分布及其对肿瘤组织病理学特征和预后的影响。这项研究包括50例接受喉切除术治疗喉鳞状细胞癌的患者，在伊斯坦布尔大学-Cerrahpaša，医学院耳鼻咽喉科，2016年1月至2018年1月病理标本采用免疫组织化学方法评估CD3、CD20、CD8、CD4、CD25和FoxP3标志物的表达,识别TIL的子组。该研究旨在揭示这些亚组如何影响肿瘤组织病理学特征和生存结果。CD3、CD20和CD4的高浸润对疾病特异性生存率有积极影响，无病生存,和无复发生存。此外,总生存期受CD3和CD4高浸润的积极影响.然而,CD8，FoxP3和CD25的表达与任何生存参数之间均无明显关系。CD3，CD20和CD4阳性细胞的浸润表明强烈的抗肿瘤免疫反应，是喉癌的有利预后因素。这些发现表明，增强CD3，CD20和CD4淋巴细胞的浸润可能是值得在临床试验中探索的治疗策略。
