RESULTS: Results showed that the level of GSH-Px were higher in the H and M groups than in the C and L groups (P < 0.05). The levels of IgM and TNF-α were higher in the M group when compared to those on the C group (P < 0.05). The level of IgA was significantly higher in the M group than in the H group (P < 0.05). Additionally, compared with the others groups, the hepatocytes in the M group displayed a radial arrangement, forming hepatic plates that were centered around the central vein. The transcriptome results revealed that proteasome 26 S subunit, ATPase 3 (PSMC3), proteasome 26 S subunit, ATPase 5 (PSMC5), proteasome 26 S subunit ubiquitin receptor, non-ATPase 4 (PSMD4), proteasome activator subunit 1 (PSME1), acyl-CoA dehydrogenase short/branched chain (ACADSB), enoyl-CoA hydratase, short chain 1 (ECHS1), serine dehydratase (SDS), ornithine transcarbamylase (OTC), and phenylalanine hydroxylase (PAH) were the hub genes regulating the amino acid metabolism in the liver.
CONCLUSIONS: In summary, dietary 18% PMK supplementation contributed to improve the hepatic phenotype, oxidative stress and immune response through regulating the expression of related genes.
结果:结果显示,H组和M组的GSH-Px水平高于C组和L组(P<0.05)。与C组相比,M组的IgM和TNF-α水平更高(P<0.05)。M组IgA程度明显高于H组(P<0.05)。此外,与其他群体相比,M组肝细胞呈放射状排列,形成以中央静脉为中心的肝板。转录组结果表明,蛋白酶体26S亚基,ATP酶3(PSMC3),蛋白酶体26S亚基,ATP酶5(PSMC5),蛋白酶体26S亚基泛素受体,非ATP酶4(PSMD4),蛋白酶体激活子亚基1(PSME1),酰基辅酶A脱氢酶短/支链(ACADSB),烯酰辅酶A水合酶,短链1(ECHS1),丝氨酸脱水酶(SDS),鸟氨酸转碳淀粉酶(OTC),和苯丙氨酸羟化酶(PAH)是调节肝脏氨基酸代谢的中心基因。
结论:总之,饮食中18%的PMK补充剂有助于改善肝脏表型,通过调节相关基因的表达来实现氧化应激和免疫应答。