RESULTS: Herein, 22 flavonoids related to quercitrin metabolism were identified based on ultra-performance liquid chromatography-electrospray ionization-tandem mass spectrometry (UPLC-ESI-MS/MS). Gas chromatography and 16S rDNA gene sequencing were used to investigate short-chain fatty acid (SCFA) content and diversity of composition of gut microbiota, respectively. The results showed that quercitrin significantly alters the beta-diversity of the gut microbiota, probiotics such as Akkermansia and Lactococcus were significantly increased, and the production of propanoate, isovalerate and hexanoate of the quercitrin group were enhanced significantly. The Spearman\'s association analysis provided evidence that Gardnerella and Akkermansia have obvious correlations with most of quercitrin metabolites and SCFAs.
CONCLUSIONS: Quercitrin and its metabolites in the colon altered the structure of the mice gut microbiota and increased the content of SCFAs. Our experiments provide valuable insights into quercitrin research and application. © 2024 Society of Chemical Industry.
结果:这里,采用超高效液相色谱-电喷雾串联质谱(UPLC-ESI-MS/MS)技术鉴定了22种与槲皮苷代谢相关的黄酮类化合物。采用气相色谱和16SrDNA基因测序技术研究短链脂肪酸(SCFA)含量和肠道菌群组成的多样性,分别。结果表明,槲皮苷显著改变肠道菌群的β-多样性,益生菌如Akkermansia和乳球菌显著增加,以及丙酸酯的生产,槲皮苷组的异戊酸和己酸均显着增强。Spearman的关联分析提供了证据,表明加德纳菌和阿克曼西亚与大多数槲皮苷代谢物和SCFA具有明显的相关性。
结论:槲皮苷及其代谢产物改变了小鼠肠道菌群的结构,增加了SCFA的含量。我们的实验为槲皮苷的研究和应用提供了有价值的见解。©2024化学工业学会。