PDF(Pubmed)
Abstract：
Introduction: Rotator cuff tear (RCT) is a common shoulder injury impacting mobility and quality of life, while traditional surgeries often result in poor healing. Tissue engineering offers a promising solution, with poly (ε-caprolactone) (PCL) being favored due to its slow degradation, biocompatibility, and non-toxicity. However, PCL lacks sufficient compression resistance. Incorporating Mg, which promotes bone growth and has antibacterial effects, could enhance RCT repair. Methods: The Mg-incorporated PCL-based scaffolds were fabricated using a 3D printing technique. The scaffolds were incorporated with different percentages of Mg (0%, 5%, 10%, 15%, and 20%). The osteogenic activities and anti-inflammatory properties of the scaffolds were evaluated in vitro using human osteoblasts and macrophages. The tissue ingrowth and biocompatibility of the scaffolds were assessed in vivo using a rat model of RCT repair. The ability of the scaffolds to enhance macrophage polarization towards the M2 subtype and inhibit inflammation signaling activation was also investigated. Results: It was found that when incorporated with 10% Mg, PCL-based scaffolds exhibited the optimal bone repairing ability in vitro and in vivo. The in vitro experiments indicated that the successfully constructed 10 Mg/PCL scaffolds enhance osteogenic activities and anti-inflammatory properties. Besides, the in vivo studies demonstrated that 10 Mg/PCL scaffolds promoted tissue ingrowth and enhanced biocompatibility compared to the control PCL scaffolds. Furthermore, the 10 Mg/PCL scaffolds enhanced the macrophages\' ability to polarize towards the M2 subtype and inhibited inflammation signaling activation. Discussion: These findings suggest that 3D-printed Mg-incorporated PCL scaffolds have the potential to improve RCT by enhancing osteogenesis, reducing inflammation, and promoting macrophage polarization. The incorporation of 10% Mg into PCL-based scaffolds provided the optimal combination of properties for RCT repair augmentation. This study highlights the potential of tissue engineering approaches in improving the outcomes of RCT repair and provides a foundation for future clinical applications.
摘要：
简介：肩袖撕裂（RCT）是一种常见的肩关节损伤，影响活动能力和生活质量，而传统手术往往导致愈合不良。组织工程提供了一个有前途的解决方案，聚（ε-己内酯）（PCL）因其降解缓慢而受到青睐，生物相容性，和无毒。然而,PCL缺乏足够的抗压缩性。加入Mg，促进骨骼生长并具有抗菌作用，可以增强RCT修复。方法：使用3D打印技术制造Mg掺入的PCL基支架。支架中掺入了不同百分比的Mg(0%，5%,10%,15%,和20%）。使用人成骨细胞和巨噬细胞在体外评估了支架的成骨活性和抗炎特性。使用RCT修复的大鼠模型在体内评估了支架的组织向内生长和生物相容性。还研究了支架增强巨噬细胞向M2亚型极化和抑制炎症信号激活的能力。结果：发现当掺入10％Mg时，基于PCL的支架在体外和体内表现出最佳的骨修复能力。体外实验表明，成功构建的10Mg/PCL支架增强了成骨活性和抗炎性能。此外,体内研究表明，与对照PCL支架相比，10Mg/PCL支架可促进组织向内生长并增强生物相容性。此外，10Mg/PCL支架增强了巨噬细胞向M2亚型极化的能力，并抑制了炎症信号激活。讨论：这些发现表明，3D打印的Mg掺入的PCL支架具有通过增强成骨作用来改善RCT的潜力，减少炎症，促进巨噬细胞极化。将10％Mg掺入PCL基支架中，为RCT修复增强提供了最佳的性能组合。这项研究强调了组织工程方法在改善RCT修复结果方面的潜力，并为未来的临床应用奠定了基础。
