PDF(Pubmed)
Abstract：
Mycotoxins, secondary metabolites produced by molds, pose significant health risk through contamination of globally consumed cereals. Ochratoxin A (OTA), a prevalent mycotoxin in cereals, is associated with various health hazards, including immunotoxicity. This study explores the bioaccessibility of OTA in bread and its impact on the gastrointestinal barrier. A focus is placed on grape pomace (GP), a by-product of the wine industry, as a potential mitigator of OTA toxicity. Results demonstrate that GP reduces OTA bioaccessibility in the human gastrointestinal system from 94% to 81% at intestinal level, showing promise in limiting the absorption of the harmful toxin. Additionally, GP exhibits cytoprotective effects, enhancing cell viability and mitigating OTA-induced toxicity in both Caco-2 and Jurkat T cells. In view of the above, to understand the mechanisms by which OTA exhibits its toxic effects, flow cytometry was chosen as the main technique for the analysis of cell cycle, reactive oxygen species levels and mitochondrial parameters. Cytofluorimetric evaluation indicates GP\'s potential in limiting OTA-induced damage at cellular level. The study suggests that GP could serve as functional ingredient to reduce mycotoxin bioaccessibility and toxicity in cereal-based foods, offering a novel and promising approach to enhance food safety and protect public health. The finding highlights the potential of utilizing grape pomace in food formulations to mitigate mycotoxin contamination, providing a valuable contribution to the ongoing efforts to ensure the safety of globally consumed cereal products.
摘要：
霉菌毒素,霉菌产生的次生代谢产物，通过污染全球消费的谷物构成重大健康风险。曲霉毒素A(OTA),谷物中普遍存在的霉菌毒素，与各种健康危害有关，包括免疫毒性.本研究探讨了面包中OTA的生物可及性及其对胃肠屏障的影响。重点放在葡萄果渣(GP)上，葡萄酒工业的副产品，作为OTA毒性的潜在缓解剂。结果表明，GP在肠道水平将人类胃肠道系统中的OTA生物可及性从94％降低到81％，显示出限制有害毒素吸收的希望。此外,GP表现出细胞保护作用，在Caco-2和JurkatT细胞中增强细胞活力并减轻OTA诱导的毒性。鉴于上述情况,为了了解OTA表现出毒性作用的机制，选择流式细胞术作为细胞周期分析的主要技术，活性氧水平和线粒体参数。细胞荧光评估表明GP在细胞水平上限制OTA诱导的损伤的潜力。该研究表明，GP可以作为功能性成分，降低谷物食品中霉菌毒素的生物可及性和毒性，提供了一种新颖而有前途的方法来增强食品安全和保护公众健康。这一发现凸显了在食品配方中利用葡萄果渣减轻霉菌毒素污染的潜力。为确保全球消费谷物产品安全的持续努力做出了宝贵贡献。
