Abstract：
Rift Valley fever (RVF) caused by Rift Valley fever virus (RVFV) is a major health concern for both domesticated animals and humans in certain endemic areas of Africa. With changing environmental conditions and identification of vectors capable of transmitting the virus, there is high risk of RVFV spreading into other parts of the world. Furthermore, unavailability of effective vaccines in the event of an outbreak can be a major challenge as witnessed recently in case of SARS-CoV2 pandemic. Hence, identifying potential vaccines and testing their protective efficacy in preclinical models before clinical testing is the absolute need of the hour. Here, we describe methods used to quantify virus-specific T cell responses in mice that were immunized with RVFV strains or antigens.
摘要：
由裂谷热病毒（RVFV）引起的裂谷热（RVF）是非洲某些流行地区驯养动物和人类的主要健康问题。随着环境条件的变化和能够传播病毒的载体的鉴定，RVFV扩散到世界其他地区的风险很高。此外，如最近在SARS-CoV2大流行的情况下所看到的那样，在爆发的情况下无法获得有效的疫苗可能是一个重大挑战。因此，在临床试验之前，确定潜在的疫苗并在临床前模型中测试其保护效力是绝对需要的。这里,我们描述了用于定量用RVFV毒株或抗原免疫的小鼠中病毒特异性T细胞应答的方法.
